PMID: 8593575Oct 30, 1995Paper

Distribution of mu-calpain proenzyme in the brain and other neural tissues in the rat

Brain Research
K OnizukaH Nishino

Abstract

We raised antibodies against the acetyl N-terminal peptide of the human mu-calpain 80 kDa (80 K) subunit (N-acetyl SEETPVYCT-GVSAQVQKQRARELG) in the rabbit. A specific antibody was purified using N-acetyl SEEITPVYCTGVSAQVQKQ peptide-conjugated Sepharose 4B as an affinity gel support. Epitope analysis revealed that the purified antibody reacted only with mu-calpain N-terminal peptides containing N-acetyl SEETT structure but no reactions occurred with other analogous peptides. Western blot analysis showed that the antibody reacted with both human and rat mu-calpain proenzymes but not with the activated calpains lacking N-terminal peptide. Using this antibody we investigated immunohistochemically the distribution of mu-calpain proenzyme in central and peripheral nervous systems as well as other non-neural tissues in the rat. The proenzyme was detected mainly in neurons both in the central and peripheral nervous tissues, but not in non-neural tissues except for red blood cells. Immunoreaction was stronger in the perikarya and/or in the nuclei than in-the cytoplasm. Specificity of the antibody was verified by an absorption test. In summary, the mu-calpain proenzyme is mainly distributed in the perikarya and/or nuclei or neurons. Our...Continue Reading

References

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Citations

Jun 8, 1999·The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi·T SaitoT Manabe
Oct 19, 2006·Journal of Neuro-oncology·Hyang-Hwa RyuSam-Suk Kang
May 1, 1998·Annals of the New York Academy of Sciences·N L BanikE L Hogan
Mar 18, 2000·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·M KunimatsuM Sasaki
Aug 23, 2003·Brain Research·Masayuki YokotaTakashi Watanabe
Jun 18, 2005·Brain Research. Brain Research Reviews·Zhao Zhong ChongKenneth Maiese

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