Distribution of N-type Ca2+ channel binding sites in rabbit brain following central administration of omega-conotoxin GVIA

European Journal of Pharmacology
S L WhorlowC E Wright

Abstract

Central administration of the N-type Ca2+ channel blocker omega-conotoxin GVIA in conscious rabbits has previously been shown to result in a slowly developing hypotensive and sympatholytic effect, with peak changes observed after 48 h. The aim of the current study was to examine the distribution of [125I] omega-conotoxin GVIA binding in rabbit brain alone or following a prior i.c.v. injection of omega-conotoxin GVIA to determine the site(s) of action of centrally administered omega-conotoxin GVIA. Brains were removed from rabbits 2 or 48 h after central administration of vehicle or non-labelled omega-conotoxin GVIA (30 pmol/kg, i.c.v.). Brain sections were then incubated with [125I] omega-conotoxin GVIA (50 pM) and the density of specific [125I] omega-conotoxin GVIA binding measured in dpm/mm2 was determined by quantitative receptor autoradiography. In the vehicle group, highest densities of [125I] omega-conotoxin GVIA binding sites (> 20 dpm/mm2) were detected in cortex, caudate, putamen, and the stratum oriens and stratum radiatum of the hippocampus. Prior (48 h) i.c.v. injection of omega-conotoxin GVIA resulted in a decrease in specific binding of [125I] omega-conotoxin GVIA, particularly in cortex and some portions of the c...Continue Reading

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Citations

Oct 14, 2003·European Journal of Pharmacology·Bruno FuksPierre Chatelain
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·M InoY Mori
Sep 23, 1997·Journal of Cardiovascular Pharmacology·C E Wright, J A Angus
Jul 13, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·X HouS Chemtob
Jun 14, 2000·Neurology·T J Guttuso

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