Distribution of ncRNAs expression across hypothalamic-pituitary-gonadal axis in Capra hircus

BMC Genomics
Emanuele CapraAlessandra Stella

Abstract

Molecular regulation of the hypothalamic-pituitary-gonadal (HPG) axis plays an essential role in the fine tuning of seasonal estrus in Capra hircus. Noncoding RNAs (ncRNAs) are emerging as key regulators in sexual development and mammalian reproduction. In order to identify ncRNAs and to assess their expression patterns, along the HPG axis, we sequenced ncRNA libraries from hypothalamus, pituitary and ovary of three goats. Among the medium length noncoding RNAs (mncRNAs) identified, small nucleolar RNAs (snoRNAs) and transfer RNAs (tRNAs) were found to be more abundant in ovary and hypothalamus, respectively. The observed GC content was representative for different classes of ncRNAs, allowing the identification of a tRNA-derived RNA fragments (tRFs) subclass, which had a peak distribution around 32-38% GC content in the hypothalamus. Differences observed among organs confirmed the specificity of microRNA (miRNA) profiles for each organ system. Data on ncRNAs in organs constituting the HPG axis will contribute to understanding their role in the physiological regulation of reproduction in goats.

References

Feb 12, 2002·Bioinformatics·Alexander SturnZlatko Trajanoski
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 12, 2005·Genomics·Julie CocquetReiner A Veitia
Sep 15, 2009·Molecular and Cellular Endocrinology·Varykina G ThackrayDjurdjica Coss
Jun 1, 2006·PLoS Biology·Richard Robinson
Jan 1, 2009·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·L McFarlane, D Wilhelm
Oct 5, 2010·Animal Reproduction Science·Alice FatetBernard Leboeuf
Sep 24, 2011·RNA·Alex BatemanChristian Zwieb
Nov 19, 2011·Nature Reviews. Genetics·Manel Esteller
Mar 13, 2012·Genome Research·Michael B ClarkJohn S Mattick
Mar 23, 2012·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·A ChristensenP Micevych
Jul 10, 2012·Genomics, Proteomics & Bioinformatics·Michael BaoJason T L Wang
Nov 10, 2012·Molecular and Cellular Endocrinology·Anthony GriecoMark R Palmert
Jul 3, 2013·Nature Reviews. Genetics·Kailiang Sun, Eric C Lai
Aug 6, 2013·Genetics and Molecular Research : GMR·Y H LingX R Zhang
Apr 1, 2014·Cell·Thomas R Cech, Joan A Steitz
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Apr 23, 2014·Molecular and Cellular Biology·Mridusmita SaikiaMaria Hatzoglou
Jun 3, 2014·BMC Biology·Irene Gallego RomeroYoav Gilad
Apr 15, 2015·Trends in Genetics : TIG·Georges St LaurentPhilipp Kapranov
Apr 17, 2015·Wiley Interdisciplinary Reviews. RNA·Fabien Dupuis-SandovalMichelle S Scott
May 20, 2015·Cell Metabolism·Sarah JinnDaniel S Ory
Aug 2, 2015·Journal of Ovarian Research·Ying LiXiaokui Yang
Aug 8, 2015·G3 : Genes - Genomes - Genetics·Jing LiDacheng Tian
Nov 11, 2015·Nucleic Acids Research·Yuk Yee LeungLi-San Wang
Feb 13, 2016·Scientific Reports·Qing LiZhi-Feng Deng
Feb 28, 2016·Nucleic Acids Research·Nicole LudwigAndreas Keller
May 14, 2016·Nucleic Acids Research·Hadi JorjaniAndreas R Gruber
Jan 21, 2017·F1000Research·Spyros Karaiskos, Andrey Grigoriev

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Citations

Nov 6, 2018·Nucleic Acids Research·UNKNOWN The RNAcentral Consortium
Mar 23, 2021·The Journal of Steroid Biochemistry and Molecular Biology·Shuwen TanHua Li

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Datasets Mentioned

BETA
SRP136431

Methods Mentioned

BETA
PCR
Assay

Software Mentioned

Fastx
Bioconductor edgeR
Toolkit collapser
Expression
Genesis
ClueGO
BLASTn
Cytoscape
Mirdeep2
EMBOSS geecee

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