Distribution of neuraminidase in Arthrobacter and its purification by affinity chromatography

Journal of Biochemistry
Y UchidaT Sugimori

Abstract

Neuraminidase [sialidase, EC 3.2.1.18] was found to be widely distributed in bacteria belonging to Arthrobacter. Among these bacteria, Arthrobacter ureafaciens, A. oxydans, and A. aurescens produced relatively potent neuraminidase activities. For the production of this enzyme, not only colominic acid, a homopolymer of N-acetylneuraminic acid, but also N-acetylneuraminic acid, the reaction product of this enzyme, are effective as sources of carbon. An affinity adsorbent specific for neuraminidase was prepared by cross-linking colominic acid with soluble starch by means of epichlorohydrin. Neuraminidase from A. ureafaciens could be purified on this affinity column. The purified neuraminidase was shown to be free from protease, N-acetylneuraminic acid aldolase, phospholipase C, and glycosidases. Aminoff's assay procedure for sialic acid was modified to avoid the centrifugation step. The modified procedure gave a higher molecular extinction coefficient.

Citations

Jan 1, 1988·The Journal of Experimental Zoology·P N SrivastavaK D Arbtan
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Dec 31, 2020·Bioorganic & Medicinal Chemistry·Jack CheesemanHelen M I Osborn

Related Concepts

Arthrobacter
Chromatography, Affinity
Enzyme Induction
Hydrolase
Neuraminidase
Carbohydrate Metabolism

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