PMID: 8584754Oct 20, 1995Paper

Distribution of phosphodiester and phosphorothioate oligonucleotides in rat brain after intraventricular and intrahippocampal administration determined by in situ hybridization

Regulatory Peptides
Y Yaida, T S Nowak

Abstract

The distribution and stability of exogenously administered oligonucleotides (oligos) are important variables determining the potential utility of antisense oligos as agents for modifying gene expression within a given brain region in vivo. In the present study, phosphodiester (PO) and phosphorothioate (PS) oligos antisense with respect to a recently cloned rat hsp70 sequence were localized in rat brain following intraventricular and intrahippocampal administration using an in situ hybridization detection method. Unlabeled PO and PS oligos were dissolved in artificial cerebrospinal fluid and infused under stereotaxic control using a syringe pump. At various intervals after administration frozen brain sections were collected on gelatin-coated slides and hybridized with 35S-labeled probe consisting of the corresponding phosphodiester sense sequence. After intraventricular administration the unmodified PO oligo exhibited a limited and strictly periventricular distribution. In contrast the PS oligo showed significant penetration into and accumulation within brain, with extensive uptake in ipsilateral striatum and dorsal hippocampus, as well as in midline periventricular structures. Both oligos remained detectable for at least two da...Continue Reading

References

Dec 1, 1992·European Journal of Pharmacology·B J ChiassonH A Robertson
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J P LeonettiB Lebleu
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S AgrawalJ Y Tang
May 1, 1991·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·T S Nowak
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S AgrawalP C Zamecnik
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E L WickstromE Wickstrom
Oct 15, 1993·Journal of Neuroscience Research·F M LongoF R Sharp
May 19, 1993·European Journal of Pharmacology·M HeiligB Söderpalm
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L WhitesellL M Neckers

❮ Previous
Next ❯

Citations

Jul 16, 2002·Pharmacology, Biochemistry, and Behavior·Marian E FundytusAndy Dray
May 10, 2003·Brain Research. Brain Research Reviews·Dirk Van OekelenJosée E Leysen
Apr 20, 2001·Brain Research. Molecular Brain Research·S P HoS P Arneric
Feb 21, 2002·Progress in Neuro-psychopharmacology & Biological Psychiatry·Alon ShamirGalila Agam
Jul 12, 2002·British Journal of Pharmacology·Reza N SharifMarian E Fundytus
Mar 24, 2015·Advanced Drug Delivery Reviews·Melvin M EversWilleke M C van Roon-Mom
Mar 29, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·J G HeckerV R Irion
Apr 7, 1999·Antisense & Nucleic Acid Drug Development·A W Szklarczyk, L Kaczmarek
Oct 9, 2004·Stroke; a Journal of Cerebral Circulation·Giuseppe PignataroLucio Annunziato
Dec 17, 2004·Pharmacological Reviews·L AnnunziatoG F Di Renzo
Jun 28, 2018·Molecular Neurobiology·Gisela ZalcmanArturo Romano
Apr 13, 2000·Cancer Control : Journal of the Moffitt Cancer Center·H H Engelhard

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.