PMID: 11918673Mar 29, 2002Paper

Distribution of phosphorylated glial fibrillary acidic protein in the mouse central nervous system

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
Masaaki TakemuraS Itohara

Abstract

Glial fibrillary acidic protein (GFAP) is the principal component of intermediate filaments (IFs) in mature astrocytes in the central nervous system (CNS). Like other IF proteins, GFAP has multiple phosphorylation sites in the N-terminal head domain. The distribution of phospho-GFAP in vivo has not been elucidated. We generated Gfap(hwt) knock-in mice, in which the coding region for the head domain of GFAP is replaced with the corresponding human sequence. In combination with a series of monoclonal antibodies (mAbs) reactive to human phospho-GFAP, we visualized the distribution of phospho-GFAP in vivo in mice. GFAP phosphorylated at Thr7, Ser8 and/or Ser13 increased postnatally in the CNS of these mice. Limited populations of GFAP-positive astrocytes were labelled with anti-phospho-GFAP mAbs in most brain areas, whereas almost all the astrocytes in the optic nerve and spinal cord were labelled. Astrocytes in the subventricular zone and rostral migratory stream preferentially contained phospho-GFAP. In a cold injury model of the cerebral cortex, we detected phospho-GFAP in reactive astrocytes at 2-3 weeks after the injury. Phospho-GFAP provides a molecular marker indicating the heterogeneity of astrocytes, and Gfap(hwt) knock-in...Continue Reading

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Citations

Jun 28, 2005·Experimental Eye Research·Akiyasu KanamoriAkira Negi
Mar 16, 2007·Neuron Glia Biology·Jason G Emsley, Jeffrey D Macklis
May 8, 2013·Glia·Daniel SunTatjana C Jakobs
Mar 23, 2012·Journal of Neurochemistry·Saadia ZahidNikhat Ahmed
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Nov 1, 2016·Molecular Biology of the Cell·Ni-Hsuan LinMing-Der Perng
Sep 15, 2019·The FEBS Journal·Holly A Rutherford, Noémie Hamilton

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