Distribution of Pico- and Nanosecond Motions in Disordered Proteins from Nuclear Spin Relaxation

Biophysical Journal
Shahid Nawaz KhanFabien Ferrage

Abstract

Intrinsically disordered proteins and intrinsically disordered regions (IDRs) are ubiquitous in the eukaryotic proteome. The description and understanding of their conformational properties require the development of new experimental, computational, and theoretical approaches. Here, we use nuclear spin relaxation to investigate the distribution of timescales of motions in an IDR from picoseconds to nanoseconds. Nitrogen-15 relaxation rates have been measured at five magnetic fields, ranging from 9.4 to 23.5 T (400-1000 MHz for protons). This exceptional wealth of data allowed us to map the spectral density function for the motions of backbone NH pairs in the partially disordered transcription factor Engrailed at 11 different frequencies. We introduce an approach called interpretation of motions by a projection onto an array of correlation times (IMPACT), which focuses on an array of six correlation times with intervals that are equidistant on a logarithmic scale between 21 ps and 21 ns. The distribution of motions in Engrailed varies smoothly along the protein sequence and is multimodal for most residues, with a prevalence of motions around 1 ns in the IDR. We show that IMPACT often provides better quantitative agreement with e...Continue Reading

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Citations

Apr 27, 2016·Journal of the American Chemical Society·Anton AbyzovMartin Blackledge
Jun 15, 2016·The Journal of Physical Chemistry Letters·Nicola SalviMartin Blackledge
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Feb 3, 2018·The Journal of Chemical Physics·Albert A SmithBeat H Meier
Jul 22, 2019·The Journal of Chemical Physics·Albert A SmithFabien Ferrage
Jan 23, 2021·PLoS Computational Biology·Giulio TeseiKresten Lindorff-Larsen
Aug 30, 2017·Biochimica Et Biophysica Acta. General Subjects·Teppei IkeyaChristian Griesinger
Mar 21, 2021·Biophysical Journal·Louise PinetCarine van Heijenoort
Dec 26, 2017·Journal of the American Chemical Society·Elise DelaforgeMalene Ringkjøbing Jensen
Aug 21, 2021·Magnetic Resonance·Timothy Crawley, Arthur G Palmer
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Nov 22, 2017·Progress in Nuclear Magnetic Resonance Spectroscopy·Nicola SalviMartin Blackledge
Nov 12, 2021·Frontiers in Molecular Biosciences·Kai Zumpfe, Albert A Smith

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Software Mentioned

NMRPipe
IMPACT
LeMaster
nlinLS

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