Abstract
The podocyte protein, 44 KD (pp44), is a podocyte-specific antigen that is selectively distributed in the cytoplasm of foot processes. It has been suggested that the pp44 antigen is associated with the cytoskeleton and helps to maintain the complex architecture of podocytes. To answer the question as to whether changes in pp44 expression are associated with changes in podocyte morphology, we investigated the distribution of the pp44 antigen in different kidney diseases. Twenty-one kidney biopsies and one nephrectomy specimen were studied by indirect immunofluorescent technique and electron microscopy. The pp44-antigen is preserved in cases associated with foot process fusion. In contrast, the antigen could not be detected in areas of capillary wall necrosis, cellular crescents or early and advanced stages of focal segmental glomerulosclerosis--even in the presence of podocytes. Our results show that the pp44 antigen is preserved in diseases associated with reversible loss of foot processes (in cases with foot process fusion associated with proteinuria). In contrast, the pp44 antigen is not detectable in the area of FSGS and cellular crescents, suggesting that in these conditions, podocytes undergo irreversible injury even if th...Continue Reading
Citations
Jan 3, 2001·Kidney International·T SrivastavaU S Alon
Aug 12, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Shengchuan DaiNan Chen
Nov 13, 2007·Nephrology·Katsuhiko AsanumaYasuhiko Tomino
Jan 14, 2004·Kidney International·Satoshi SasakiKunihiko Kobayashi
Jul 31, 2009·Experimental & Molecular Medicine·Won-Ho HahnSung-Do Kim
Apr 17, 2008·Critical Reviews in Clinical Laboratory Sciences·Vladimír Tesar, Tomás Zima
Mar 6, 2002·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Prasad Devarajan, Adrian Spitzer
Mar 28, 2006·Life Sciences·Makoto HirakawaMitsuo Iida
Dec 21, 2013·Lupus·G M RezendeE Bonfa
Feb 16, 2011·Experimental Biology and Medicine·Lina JiangQingfeng Fan
Jul 15, 2005·American Journal of Physiology. Renal Physiology·Krikor BijianAndrey V Cybulsky
Oct 10, 2008·American Journal of Physiology. Renal Physiology·Andrej SkoberneMario Schiffer
Feb 9, 2000·American Journal of Physiology. Renal Physiology·H Pavenstädt
Jan 16, 1999·Journal of the American Society of Nephrology : JASN·L BarisoniV D'Agati