PMID: 6166618Jun 1, 1981Paper

Distribution of poly(A)-containing RNA during normal pollen development and during induced pollen embryogenesis in Hyoscyamus niger

The Journal of Cell Biology
V Raghavan

Abstract

The distribution of poly(A)-containing RNA [poly(A)+RNA] in pollen grains of Hyoscyamus niger during normal gametophytic development and embryogenic development induced by culture of anther segments was followed by in situ hybridization with [3H]-polyuridylic acid as a probe. No binding of the isotope occurred in pollen grains during the uninucleate phase of their development. Although [3H]polyuridylic acid binding sites were present in the generative and vegetative cells of maturing pollen grains, they almost completely disappeared from mature grains ready to germinate. During pollen germination, poly(A)+RNA formation was transient and was due to the activity of the generative nucleus, whereas the vegetative nucleus and the sperm cells failed to interact with the applied probe. In cultured anther segments, moderate amounts of poly(A)+RNA were detected in the uninucleate, nonvacuolate, embryogenically determined pollen grains. Poly(A)+RNA accumulation in these grains was sensitive to actinomycin D, suggesting that it represents newly transcribed mRNA. After the first haploid mitosis in the embryogenically determined pollen grains, only those grains in which the generative nucleus alone or along with the vegetative nucleus accum...Continue Reading

References

May 1, 1977·The Journal of Cell Biology·V Raghavan
Mar 19, 1973·Biochimica Et Biophysica Acta·H Bultmann, C D Laird
Jan 1, 1974·Annual Review of Biochemistry·G Brawerman
Feb 1, 1973·The Journal of Cell Biology·M L PolanW Gehring
May 5, 1974·Journal of Molecular Biology·A Shenkin, R H Burdon
Sep 30, 1970·Biochemical and Biophysical Research Communications·B S FanslerJ Schultz
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M Blumenfled, H S Forrest
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J E DarnellR J Tushinski
Apr 1, 1970·Developmental Biology·J P Mascarenhas, E Bell
Oct 1, 1966·Experimental Cell Research·D M Steffensen

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