Distribution of prepubertal and adult goat oocyte cortical granules during meiotic maturation and fertilisation: ultrastructural and cytochemical study

Molecular Reproduction and Development
Esther VelillaMaria-Teresa Paramio

Abstract

The aim of this study was evaluate cortical granule (CG) distribution during in vitro maturation (IVM) and fertilisation of prepubertal goat oocytes compared to CG distribution of ovulated and in vitro fertilised oocytes from adult goats. Oocytes from prepubertal goats were recovered from a slaughterhouse and were matured in M199 with hormones and serum for 27 hr. Ovulated oocytes were collected from gonadotrophin treated Murciana goats. Frozen-thawed spermatozoa were selected by centrifugation in percoll gradient and were capacitated in DMH with 20% steer serum for 1 hr. Ovulated and IVM-oocytes were inseminated in DMH medium with steer serum and calcium lactate for 20 hr. Oocytes and presumptive zygotes were stained with FITC-LCA (Lens culinaris agglutinin labelled with fluorescein isothiocyanate) and observed under a confocal laser scanning microscope. Ultrastructure morphology of oocytes and presumptive zygotes were analysed by transmission electron microscopy (TEM). Prepubertal goat oocytes at germinal vesicle stage show a homogeneous CG distribution in the cytoplasm. IVM-oocytes at Metaphase II (MII) and ovulated oocytes presented CGs located in the cortex with the formation of a monolayer beneath to the plasma membrane. ...Continue Reading

References

Mar 1, 1975·Biology of Reproduction·B G Brackett, G Oliphant
Nov 1, 1992·Biology of Reproduction·A P ByersD E Wildt
Oct 1, 1987·Gamete Research·N CrozetP Chemineau
Dec 1, 1994·Molecular Reproduction and Development·T Hoodbhoy, P Talbot
Mar 1, 1994·Molecular Reproduction and Development·R J AsseyB Purwantara
Dec 1, 1996·Molecular Reproduction and Development·P DamianiR T Duby
Mar 23, 2000·Theriogenology·M J PalomoM T Paramio
May 1, 2001·Theriogenology·D T Armstrong
Aug 18, 2001·Zygote : the Biology of Gametes and Early Embryos·P VillamedianaM T Paramio
Nov 2, 2002·Molecular Reproduction and Development·Gustavo F CarneiroBarry A Ball
Jan 1, 1994·Theriogenology·A MartinoM T Paramio
Jan 15, 1995·Theriogenology·A MartinoM T Paramio

❮ Previous
Next ❯

Citations

Jun 1, 2011·PloS One·Michael EitelBernd Schierwater
Nov 26, 2014·Reproductive Biology and Endocrinology : RB&E·Maria G PalmeriniGuido Macchiarelli
Jan 26, 2007·Animal Reproduction Science·Begoña AnguitaDolors Izquierdo
Oct 4, 2014·Reproduction in Domestic Animals = Zuchthygiene·M-T Paramio, D Izquierdo
Jun 29, 2010·American Journal of Obstetrics and Gynecology·Shan LiuZi J Chen
Dec 9, 2009·Reproduction in Domestic Animals = Zuchthygiene·M Arias-ÁlvarezP L Lorenzo
Nov 30, 2013·Zygote : the Biology of Gametes and Early Embryos·J López-SaucedoM T Paramio
Sep 21, 2017·Biology·Karen L ReaderJennifer L Juengel
Jul 3, 2021·Cells·Antonella MastroroccoMaria Elena Dell'Aquila

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.