Distribution of SARS-CoV-2 PCR Cycle Threshold Values Provide Practical Insight Into Overall and Target-Specific Sensitivity Among Symptomatic Patients

American Journal of Clinical Pathology
Blake W BuchanNathan A Ledeboer

Abstract

We examined the distribution of reverse transcription polymerase chain reaction (RT-PCR) cycle threshold (CT) values obtained from symptomatic patients being evaluated for coronavirus disease 2019 (COVID-19) to determine the proportion of specimens containing a viral load near the assay limit of detection (LoD) to gain practical insight to the risk of false-negative results. We also examined the relationship between CT value and patient age to determine any age-dependent difference in viral load or test sensitivity. We collected CT values obtained from the cobas severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) assay corresponding to 1,213 combined nasopharyngeal-oropharyngeal specimens obtained from symptomatic individuals that were reported as positive or presumptive positive for SARS-CoV-2. CT values were stratified by SARS-CoV target and patient age group. In total, 93.3% to 98.4% of specimens demonstrated CT values greater than 3× the assay LoD, at which point false-negative results would not be expected. The mean of CT values between age groups was statistically equivalent with the exception of patients in age group 80 to 89 years, which demonstrated slightly lower CTs. Based on the distribution of observed CT ...Continue Reading

References

Jan 31, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·X L PangUNKNOWN Canadian Society of Transplantation
Jul 9, 2010·Clinical Microbiology Reviews·Eileen M Burd
Mar 12, 2020·JAMA : the Journal of the American Medical Association·Wenling WangWenjie Tan
Apr 2, 2020·European Journal of Radiology·Chunqin LongHonglu Li
Apr 3, 2020·Nature·Roman WölfelClemens Wendtner
Apr 22, 2020·The Journal of Infection·Grace LuiPaul Ks Chan
Apr 26, 2020·Journal of Clinical Microbiology·Wei ZhenGregory J Berry
Jun 4, 2020·The New England Journal of Medicine·Yuan-Po TuEthan M Berke
Jun 23, 2020·Respiratory Medicine Case Reports·Kathleen J RamosAlexander Greninger
Jun 26, 2020·Journal of Oral Science·Yoshimasa TakeuchiRyutaro Kobayashi

❮ Previous
Next ❯

Citations

Sep 30, 2020·Diagnostics·Arunkumar ArumugamSeason Wong
Oct 18, 2020·American Journal of Clinical Pathology·Namhee KimSue Shin
Apr 25, 2020·The New England Journal of Medicine·Rajesh T GandhiCarlos Del Rio
Feb 13, 2021·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Anne Carroll, Eleanor McNamara
Apr 21, 2021·Experimental Gerontology·Justyna Mikuła-Pietrasik, Krzysztof Książek
Aug 5, 2021·Journal of Clinical Microbiology·Lao-Tzu Allan-Blitz, Jeffrey D Klausner
Aug 7, 2021·Clinical Otolaryngology : Official Journal of ENT-UK ; Official Journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery·Mohammad Hosein Taziki BalajeliniSeyed Mehran Hosseini
Jun 25, 2021·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Richard D SmithSurbhi Leekha
Sep 2, 2021·Journal of the Pediatric Infectious Diseases Society·Laura FordHannah L Kirking
Jul 3, 2021·Journal of Clinical Laboratory Analysis·Kimberly NewsomPetr Starostik
Sep 25, 2021·Clinical Chemistry·Daniel S HermanThomas J S Durant
Oct 5, 2021·STAR Protocols·Roberto AlcántaraPohl Milón
Jul 22, 2021·The Journal of Applied Laboratory Medicine·Saravanan RajuBijal A Parikh

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
lavage

Software Mentioned

Excel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.