Distribution of Sertoli cell microtubules, microtubule-dependent motors, and the Golgi apparatus before and after tight junction formation in developing rat testis

Microscopy Research and Technique
D M RedenbachK Boekelheide

Abstract

Sertoli cells are polarized epithelial cells of the seminiferous epithelium which provide structural and physiological support for differentiating germ cells. They establish different basal and adluminal environments for the selective nurturing of pre- and post-meiotic germ cells within the seminiferous epithelium, segregated by the Sertoli-Sertoli cell tight junctional complex, the blood-testis barrier. Tight junction formation between epithelial cells in vitro is a critical polarizing event associated with changes in polarized targeting of membrane-specific proteins and reorganization of microtubules, centrioles, and the Golgi apparatus. To investigate whether tight junction formation is associated with organelle reorganization in Sertoli cells in vivo, we have characterized distribution patterns of Sertoli cell microtubules, the mechanoenzymes kinesin and cytoplasmic dynein, and the Golgi apparatus during tight junction formation in developing rat testis. Immunocytochemistry on samples taken at 5, 10, 15, 20, and 25 days of age was used to examine the distribution of these proteins during the extensive cellular reorganization that culminates in the formation of the blood-testis barrier at 19 days of age. Our data show that t...Continue Reading

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Citations

Dec 10, 2002·Annual Review of Pharmacology and Toxicology·Kim BoekelheideStephanie Thompson
Jan 21, 2014·Seminars in Cell & Developmental Biology·Liza O'Donnell, Moira K O'Bryan
Feb 17, 2017·Biology of Reproduction·Dan-Dan MaWan-Xi Yang
Jul 18, 2018·American Journal of Physiology. Endocrinology and Metabolism·Qing WenC Yan Cheng
Apr 17, 2019·Molecular Reproduction and Development·Jiarui SunEndong Bao

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