Distribution of T Cells in Rainbow Trout (Oncorhynchus mykiss) Skin and Responsiveness to Viral Infection

PloS One
Esther LealC Tafalla

Abstract

Although the skin constitutes the first line of defense against waterborne pathogens, there is a great lack of information regarding the skin associated lymphoid tissue (SALT) and whether immune components of the skin are homogeneously distributed through the surface of the fish is still unknown. In the current work, we have analyzed the transcription of several immune genes throughout different rainbow trout (Oncorhynchus mykiss) skin areas. We found that immunoglobulin and chemokine gene transcription levels were higher in a skin area close to the gills. Furthermore, this skin area as well as other anterior sections also transcribed significantly higher levels of many different immune genes related to T cell immunity such as T cell receptor α (TCRα), TCRγ, CD3, CD4, CD8, perforin, GATA3, Tbet, FoxP3, interferon γ (IFNγ), CD40L and Eomes in comparison to posterior skin sections. In agreement with these results, immunohistochemical analysis revealed that anterior skin areas had a higher concentration of CD3(+) T cells and flow cytometry analysis confirmed that the percentage of CD8(+) T lymphocytes was also higher in anterior skin sections. These results demonstrate for the first time that T cells are not homogeneously distribu...Continue Reading

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Citations

Feb 27, 2017·Developmental and Comparative Immunology·Irene Salinas, Susana Magadán
Nov 1, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ali SepahiIrene Salinas
Mar 21, 2018·Nature Reviews. Immunology·Martin F Flajnik
Jan 5, 2019·Current Pharmaceutical Design·Thea MagroneEmilio Jirillo
Jan 6, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Estefanía Muñoz-AtienzaCarolina Tafalla
May 8, 2019·Scientific Reports·Deepti M PatelMonica F Brinchmann
Jul 29, 2020·Science China. Life Sciences·Yaoguo LiJun Zou
May 31, 2017·Frontiers in Immunology·Cecelia Kelly, Irene Salinas

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