PMID: 2492045Feb 1, 1989Paper

Distribution of tau proteins in the normal human central and peripheral nervous system

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
J Q TrojanowskiV M Lee

Abstract

In human brain, antibodies to tau proteins primarily label abnormal rather than normal structures. This might reflect altered immunoreactivity owing to post-mortem proteolysis, disease, or species differences. We addressed this issue by comparing the distribution of tau in bovine and human post-mortem nervous system tissues and in human neural cell lines, using new monoclonal antibodies (MAb) specific for phosphate-independent epitopes in bovine and human tau. In neocortex, hippocampus, and cerebellum, immunoreactive tau was widely expressed but segregated into the axon-neuropil domain of neurons. In spinal cord and peripheral nervous system, tau immunoreactivity was similarly segregated but less abundant. No immunoreactive tau was detected with our MAb in glial cells or in human neural cell lines that express neurofilament or glial filament proteins. Post-mortem delays in tissue denaturation of less than 24 hr did not affect the distribution of tau, but the method used to denature tissues did, i.e., microwave treatment preserved tau immunoreactivity more effectively than chemical fixatives such as Bouin's solution, and formalin-fixed tissue samples reacted poorly with our anti-tau MAb. We conclude that the distribution of tau ...Continue Reading

Citations

Jan 1, 1990·Cell Motility and the Cytoskeleton·G Lee
Jan 1, 1994·Cell Motility and the Cytoskeleton·R Brandt, G Lee
Mar 1, 1990·Journal of Neuroscience Research·H Ksiezak-RedingS H Yen
Mar 1, 1990·Journal of Neuroscience Research·H Ksiezak-RedingS H Yen
Jan 1, 1993·Molecular Neurobiology·L C Doering
Mar 20, 2010·Acta Neuropathologica·Zahra Al-Hajri, Marc R Del Bigio
Dec 1, 1994·Journal of Neuroscience Methods·G R Login, A M Dvorak
Nov 1, 1993·Brain Research. Molecular Brain Research·L A SygowskiC B Caputo
May 1, 1995·Neurobiology of Aging·J W Mandell, G A Banker
Jan 1, 1994·Neurobiology of Aging·Y T LiJ Q Trojanowski
Mar 1, 1996·Neurobiology of Aging·D S Woodruff-Pak, J Q Trojanowski
Mar 17, 1997·Brain Research. Developmental Brain Research·T D VeenstraR Kumar
Feb 17, 2010·Nature Reviews. Neurology·Kaj BlennowHenrik Zetterberg
Feb 13, 2013·Nature Reviews. Neurology·Henrik ZetterbergKaj Blennow
Jan 6, 2000·The Biochemical Journal·S M JenkinsG V Johnson
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S S Matsuyama, L F Jarvik
Mar 11, 1990·Proceedings of the National Academy of Sciences of the United States of America·H AraiJ Q Trojanowski
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S E ArnoldJ Q Trojanowski
Jan 22, 2010·Human Molecular Genetics·Chenere P RamseyBenoit I Giasson
Oct 1, 1991·The European Journal of Neuroscience·Beat M. Riederer, Giorgio M. Innocenti
Oct 23, 2009·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·M JonssonA Wallin
Jan 17, 1996·Annals of the New York Academy of Sciences·V M Lee
Jun 24, 2010·BMC Neurology·Niklas MattssonLars Hagberg
Dec 24, 2009·BMC Neurology·Magnus GisslénHenrik Zetterberg
Dec 3, 2010·Alzheimer's Research & Therapy·Henrik ZetterbergBob Olsson
Feb 10, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Niklas Mattsson
Aug 25, 2012·Biomarkers in Medicine·Henrik ZetterbergSanna-Kaisa Herukka
Jan 15, 2014·Journal of Neural Transmission·Rolf AnckarsäterHenrik Zetterberg
Jan 12, 2005·The Journal of Cell Biology·Edward B LeeVirginia M-Y Lee
Jan 28, 2016·Journal of Molecular Neuroscience : MN·Yanina Ivashko PachimaIllana Gozes
Dec 15, 2015·Human Brain Mapping·Timothy B MeierAndrew R Mayer
Oct 3, 2012·European Journal of Paediatric Neurology : EJPN : Official Journal of the European Paediatric Neurology Society·Pashtun ShahimNiklas Mattsson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: Tau & TDP-43

Alzheimer's disease is a neurodegenerative disease. This feed focuses on the underlying role of tau proteins and TAR DNA-binding protein 43, as well as other genetic factors, in Alzheimer's disease.