Distribution of terminal sugar residues in the testis of the spotted ray Torpedo marmorata

Molecular Reproduction and Development
Annamaria LiguoroPiero Andreuccetti

Abstract

Lectins represent a class of proteins/glycoproteins binding specifically to terminal sugar residues. The present investigation aims to identify lectin-binding sites in testis of Torpedo marmorata. Using a panel of lectins coupled with fluoresceine isothiocyanate, we demonstrated that germ and somatic cells present in Torpedo testis contain glycoconjugates, whose distribution at the level of the surface, the cytoplasm and the nucleus changes during germ cell differentiation. Moreover our observations demonstrate that the germ cells undergoing apoptosis (Prisco et al., 2003a: Mol Reprod Dev 64:341-348) overexpress a residual sugar recognised by WFA lectin that can be considered a specific marker for apoptotic germ cells. Finally, our results indicate that there is a progressive increase in glycosilation during spermatogenesis, especially at the level of the acrosome in the spermatocyte-spermatid step, and that Leydig cells are differently stained in relation to the spermatogenetic cycle.

References

Oct 1, 1991·The Journal of Veterinary Medical Science·M KurohmaruY Hayashi
Jul 1, 1985·Journal of Andrology·M Arya, T Vanha-Perttula
Mar 1, 1989·Journal of Cellular Physiology·M Ishihara, H E Conrad
Jan 1, 1989·The Journal of Experimental Zoology. Supplement : Published Under Auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry·G CallardM E Cuevas
May 1, 1989·Journal of Cellular Physiology·N S FedarkoH E Conrad
Apr 1, 1986·The American Journal of Anatomy·M Arya, T Vanha-Perttula
Jul 1, 1985·The Anatomical Record·M C Lee, I Damjanov
Jan 1, 1966·Zeitschrift für Zellforschung und mikroskopische Anatomie·H P Stanley
Jan 1, 1984·Differentiation; Research in Biological Diversity·M C Lee, I Damjanov
Feb 11, 1999·International Journal of Andrology·M I ArenasR Paniagua
May 20, 1999·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·W C HamlettR Spieler
Mar 11, 2000·Reviews of Reproduction·T M RowlandsO W Blaschuk
Oct 6, 2000·Research in Veterinary Science·A Verini-SuppliziF Parillo
Nov 18, 2000·Histochemistry and Cell Biology·I TaniiK Toshimori
Aug 31, 2002·Molecular Reproduction and Development·Prisco MarinaAngelini Francesco
Jan 28, 2003·Molecular Reproduction and Development·Marina PriscoPiero Andreuccetti
Aug 1, 1963·The Journal of Endocrinology·T H SIMPSONH GOTTFRIED
Nov 1, 1964·The Journal of Endocrinology·T H SIMPSONS V HUNT

❮ Previous
Next ❯

Citations

Aug 24, 2010·Zygote : the Biology of Gametes and Early Embryos·Sérgio Fonseca ZaidenElisabeth Criscuolo Urbinati
Dec 19, 2012·Marine Drugs·Alessandra Gallo, Maria Costantini
Sep 7, 2006·Molecular Reproduction and Development·Marina PriscoPiero Andreuccetti
Apr 18, 2009·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Marina PriscoPiero Andreuccetti
May 13, 2011·Microscopy Research and Technique·Galder ValbuenaFrancisco José Sáez
Jul 7, 2021·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Mahmoud S GewailyMohamed Kassab

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis