Distribution of tetrodotoxin in the ribbon worm Lineus alborostratus (Takakura, 1898) (nemertea): Immunoelectron and immunofluorescence studies

Toxicon : Official Journal of the International Society on Toxinology
Timur Yu MagarlamovAlexey V Chernyshev

Abstract

Transmission electron and confocal laser scanning (CLSM) microscopies with monoclonal anti-tetrodotoxin antibodies were used to locate tetrodotoxin (TTX) in tissues and gland cells of the ribbon worm Lineus alborostratus. CLSM studies have shown that the toxin is primarily localized in the cutis (special subepidermal layer) of the body wall and in the glandular epithelium of the proboscis. Immunoelectron micrographs have shown that only subepidermal bacillary gland cells type I in cutis and pseudocnidae-containing and mucoid gland cells manifested TTX-gold labeling. TTX was associated with the nuclear envelope, endoplasmic reticulum membrane, and secretory granules of TTX-positive gland cells. These studies indicate that ТТХ is brought into the cytoplasm of the glandular cells of the cutis and proboscis epithelium, where it is associated with membrane-enclosed organelles involved in protein secretion and then concentrated in glandular granules.

References

Jan 1, 1990·Toxicon : Official Journal of the International Society on Toxinology·A E AliK Hashimoto
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·K MiyazawaK Hashimoto
Jan 1, 1987·Toxicon : Official Journal of the International Society on Toxinology·K MiyazawaK Hashimoto
Mar 1, 2000·Toxicon : Official Journal of the International Society on Toxinology·M AsakawaK Miyazawa
Oct 17, 2001·Toxicon : Official Journal of the International Society on Toxinology·M B TanuT Noguchi
Nov 2, 2001·Toxicon : Official Journal of the International Society on Toxinology·Kazumi TsurudaTamao Noguchi
Dec 7, 2002·Toxicon : Official Journal of the International Society on Toxinology·Yahia MahmudTamao Noguchi
Jun 2, 2009·Toxicon : Official Journal of the International Society on Toxinology·Becky L Williams, Roy L Caldwell
Sep 26, 2009·Toxicon : Official Journal of the International Society on Toxinology·Takuya MatsumotoYuji Nagashima
Feb 20, 2010·Toxicon : Official Journal of the International Society on Toxinology·Dietrich MebsMari Yotsu-Yamashita
Apr 7, 2012·Toxicon : Official Journal of the International Society on Toxinology·Dietrich MebsOsamu Arakawa

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.