Distribution, recovery and concentration of platelets and leukocytes in L-PRP prepared by centrifugation

Colloids and Surfaces. B, Biointerfaces
Bruna Alice Gomes de MeloMaria Helena Andrade Santana

Abstract

Platelet-rich plasma (PRP) is an autologous product prepared from whole blood (WB) that is widely used in regenerative medicine. In clinical practice, discontinuous centrifugation is used for both hand- and machine-prepared PRP. However, separation of WB fractions via centrifugation is a complex process, and the lack of clear mechanisms limits the understanding and evaluation of PRP preparation methods This paper focuses on the distribution, recovery and concentration factor of platelets and leukocytes in L-PRP (leukocyte and platelet-rich plasma) to define a concentration pattern for these blood components due to centrifugation conditions. WB collected from three healthy donors was centrifuged for 10min at 50-800 xg in a first step and then at 400 xg in a second step. The results from the first centrifugation step showed most platelets to be distributed in the upper layer (UL) and the buffy coat (BC), with approximately 14.5±5.2% retained in the bottom layer (BL). Most leukocytes were present in the BL. The greatest platelet recoveries from L-PRP were obtained at up to 150 xg (88.5±16.9%). The cumulative concentration factors with respect to the WB from the second centrifugation step were 6 and 1.2 for platelets and leukocytes...Continue Reading

Citations

Jan 30, 2019·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ju TianBiao Cheng
Jul 31, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bruna Alice Gomes de MeloMaria Helena Andrade Santana
Sep 20, 2020·Acta Biomaterialia·Bruna A G de MeloMarimelia A Porcionatto
Mar 13, 2021·World Journal of Stem Cells·José Fábio Santos Duarte LanaMarco Antonio Percope de Andrade
Aug 14, 2021·Journal of Clinical Orthopaedics and Trauma·Lucas da FonsecaJosé Fábio Lana
Sep 22, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Susanne Clemen CapionJens Rikardt Andersen

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