Disturbed shear stress reduces Klf2 expression in arterial-venous fistulae in vivo

Physiological Reports
Kota YamamotoAlan Dardik

Abstract

Laminar shear stress (SS) induces an antiproliferative and anti-inflammatory endothelial phenotype and increases Klf2 expression. We altered the diameter of an arteriovenous fistula (AVF) in the mouse model to determine whether increased fistula diameter produces disturbed SS in vivo and if acutely increased disturbed SS results in decreased Klf2 expression. The mouse aortocaval fistula model was performed with 22, 25, or 28 gauge needles to puncture the aorta and the inferior vena cava. Duplex ultrasound was used to examine the AVF and its arterial inflow and venous outflow, and SS was calculated. Arterial samples were examined with western blot, immunohistochemistry, and immunofluorescence analysis for proteins and qPCR for RNA. Mice with larger diameter fistulae had diminished survival but increased AVF patency. Increased SS magnitudes and range of frequencies were directly proportional to the needle diameter in the arterial limb proximal to the fistula but not in the venous limb distal to the fistula, with 22-gauge needles producing the most disturbed SS in vivo. Klf2 mRNA and protein expression was diminished in the artery proximal to the fistula in proportion to increasing SS. Increased fistula diameter produces increased...Continue Reading

References

Nov 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·L W KraissA W Clowes
Jun 8, 2002·Annals of Biomedical Engineering·Brian P Helmke, Peter F Davies
Sep 18, 2002·Kidney International·Michael Allon, Michelle L Robbin
May 12, 2004·The Journal of Experimental Medicine·Sucharita SenBanerjeeMukesh K Jain
Jun 5, 2004·Trends in Cardiovascular Medicine·Shannon McCueB Lowell Langille
Feb 10, 2006·Biochemical and Biophysical Research Communications·Nanping WangShu Chien
Apr 6, 2006·Journal of Internal Medicine·S LehouxA Tedgui
Oct 3, 2006·Journal of Vascular Surgery·Deborah AbelesGuillermo García-Cardeña
Nov 14, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Shu Chien
Jun 6, 2009·Journal of Vascular Surgery·Lannery S LauvaoJoseph L Mills
Aug 18, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Douglas NamHanjoong Jo
Oct 6, 2010·Antioxidants & Redox Signaling·Lalitha NayakMukesh K Jain
Nov 6, 2010·Antioxidants & Redox Signaling·Patrizia NigroBradford C Berk
Jul 21, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Bogdan Ene-Iordache, Andrea Remuzzi
Nov 11, 2011·Journal of Vascular Surgery·George E SmithIan C Chetter
Dec 6, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Ji Zhang, Morton H Friedman
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ding-Yu LeeJeng-Jiann Chiu
Feb 1, 2013·Journal of Biomechanical Engineering·Patrick M McGahAlberto Aliseda
Apr 2, 2013·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Tonia C RothuizenJoris I Rotmans
Jul 31, 2013·Journal of Visualized Experiments : JoVE·Kota YamamotoAlan Dardik
Oct 8, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Kota YamamotoAlan Dardik
Mar 4, 2014·The Journal of Surgical Research·Daniel Y LuAlan Dardik
Apr 23, 2014·Progress in Molecular Biology and Translational Science·Peter Novodvorsky, Timothy J A Chico
Sep 19, 2014·European Heart Journal·Brenda R KwakPaul C Evans
Oct 14, 2014·The Open Urology & Nephrology Journal·Michelle K FittsYan-Ting Shiu

❮ Previous
Next ❯

Citations

Dec 25, 2015·Annals of Biomedical Engineering·Michela BozzettoAndrea Remuzzi
Jan 27, 2017·International Urology and Nephrology·Aleksandar JankovicNada Dimkovic
Mar 16, 2017·The Journal of Vascular Access·Andrea RemuzziPaolo Brambilla
Dec 10, 2015·Antioxidants & Redox Signaling·Joan Fernández EsmeratsHanjoong Jo
Oct 27, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Marco FranzoniAndrea Remuzzi
Nov 15, 2016·Antioxidants & Redox Signaling·Stefanie KohlgrüberJoachim Altschmied
Feb 26, 2019·International Heart Journal·Shun MinatsukiIssei Komuro
Jul 1, 2017·Cardiovascular Engineering and Technology·Bogdan Ene-Iordache, Andrea Remuzzi
Jul 29, 2017·Cardiovascular Engineering and Technology·Andrea Remuzzi, Michela Bozzetto
Feb 6, 2021·Journal of Vascular Surgery. Vascular Science·Ryosuke TaniguchiAlan Dardik
Jan 1, 2020·Journal of Vascular Surgery. Vascular Science·Yutaka MatsubaraAlan Dardik
Aug 24, 2021·Journal of Vascular Surgery. Vascular Science·Shin-Rong LeeAlan Dardik

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

Prism
PictureFrame
Photoshop
Windows
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Visualized Experiments : JoVE
Kota YamamotoAlan Dardik
American Journal of Physiology. Heart and Circulatory Physiology
Kota YamamotoAlan Dardik
European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery
C J van KeulenJ A Rauwerda
© 2021 Meta ULC. All rights reserved