Disulfide bond assignment of the Ebola virus secreted glycoprotein SGP

Biochemical and Biophysical Research Communications
Laura G BarrientosAnthony Sanchez

Abstract

The non-structural glycoprotein (SGP) of Ebola virus (EboV) is secreted in large amounts from infected cells as a disulfide-linked homodimer. In this communication, highly purified SGP, derived from Vero E6 cultures infected with the Zaire species of EboV, was used to determine the correct localization of inter- and intrachain disulfide bonds. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry analysis of proteolytic cleavage fragments indicates that all cysteines (six per monomeric unit) form unique disulfide bonds. Monomers of the SGP homodimer are joined in a parallel manner by two intersubunit disulfide bonds formed between paired N-terminal and C-terminal cysteines (C53-C53' and C306-C306'). The remaining cysteines are involved in intrachain disulfide bonding (paired as C108-C135 and C121-C147), which resembles the disulfide bond topology of fibronectin type II domains. The findings presented here provide the foundation for future studies aimed at defining the structural and functional properties of SGP.

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Citations

Oct 19, 2011·The Journal of Infectious Diseases·Ayaka IwasaYoshihiro Kawaoka
Mar 18, 2011·Journal of Virology·Masfique MehediHeinz Feldmann
Jun 24, 2011·Journal of Virology·Sheli R RadoshitzkyJens H Kuhn
Dec 29, 2012·PLoS Pathogens·Gopi S MohanChinglai Yang
Jan 1, 2009·Future Virology·Jeffrey E Lee, Erica Ollmann Saphire
Oct 31, 2015·Viruses·Nathan H Vande BurgtPaul Bates
Nov 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Charles D MurinErica Ollmann Saphire
Jul 4, 2015·The Journal of Infectious Diseases·Valentina A VolchkovaViktor E Volchkov
Jul 31, 2007·Virology·Darryl FalzaranoHeinz Feldmann
Oct 30, 2014·Viral Immunology·Marc-Antoine de La VegaXiangguo Qiu
Aug 27, 2016·Nature Microbiology·Jesper PallesenAndrew B Ward
Nov 18, 2016·Virologica Sinica·Yun-Jia NingHualin Wang
Sep 16, 2006·Chembiochem : a European Journal of Chemical Biology·Darryl FalzaranoHeinz Feldmann
Jun 8, 2021·Biochemical and Biophysical Research Communications·Douyue LiZhongyang Tan

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