PMID: 8443183Mar 9, 1993Paper

Disulfide bonds in recombinant human platelet-derived growth factor BB dimer: characterization of intermolecular and intramolecular disulfide linkages

Biochemistry
M HaniuW C Kenney

Abstract

Interchain cystines of PDGF-BB dimer were characterized by Edman reaction and by SDS-PAGE analysis on the protein which was chemically cleaved at Trp-40. It was found that Cys-43 has a key role in dimer formation, asymmetrically cross-linked to a cysteine residue of another identical subunit. The remaining cystines participate in the intramolecular disulfide linkages. Pepsin digestion of PDGF-BB dimer generated several small peptides and one ubiquitous Cys-containing peptide. Sequence analyses of several Cys-containing peptides indicated the existence of three intramolecular disulfide linkages including Cys-16--Cys-60, Cys-49--Cys-97, and Cys-53--Cys-99. Two interchain disulfide bonds of Cys-43--Cys-52 between two subunits were deduced from the partial reduction and alkylation of PDGF-BB. This study provides chemically determined disulfide linkages of PDGF-BB.

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H N AntoniadesC D Stiles
Jul 1, 1991·Analytical Biochemistry·E Canova-DavisV T Ling
Jun 1, 1991·International Journal of Peptide and Protein Research·B N ViolandE W Kolodziej
Apr 4, 1989·Biochemistry·S Vogel, J Hoppe
Jan 1, 1985·Current Topics in Cellular Regulation·T F DeuelJ S Huang
Jul 18, 1986·Cell·R RossD F Bowen-Pope
May 1, 1984·Cell·C H Heldin, B Westermark
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C H HeldinA Wasteson

❮ Previous
Next ❯

Citations

Sep 18, 1998·Biochimica Et Biophysica Acta·C H HeldinL Rönnstrand
Mar 29, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·C RoitschD Malarme
Dec 22, 1999·Biochemical and Biophysical Research Communications·A ScaloniP Pelosi
Jun 11, 2014·The Journal of Oral Implantology·Prasun ShahJames Rutkowski
Nov 18, 2005·Biochemical and Biophysical Research Communications·Erawan Borkham-KamphorstRalf Weiskirchen
Jul 6, 2014·Analytical Chemistry·Chia-Wei Wang, Huan-Tsung Chang
Oct 6, 2000·The Journal of Biological Chemistry·J LiW W Young
Feb 25, 2003·The Journal of Biological Chemistry·Laila J ReigstadJohan R Lillehaug
Oct 3, 1999·Physiological Reviews·C H Heldin, B Westermark

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.