Disulfide-dependent self-assembly of adiponectin octadecamers from trimers and presence of stable octadecameric adiponectin lacking disulfide bonds in vitro.

Biochemistry
David B BriggsTsu-Shuen Tsao

Abstract

Adiponectin is a circulating insulin-sensitizing hormone that homooligomerizes into trimers, hexamers, and higher molecular weight (HMW) species. Low levels of circulating HMW adiponectin appear to increase the risk for insulin resistance. Currently, assembly of adiponectin oligomers and, consequently, mechanisms responsible for decreased HMW adiponectin in insulin resistance are not well understood. In the work reported here, we analyzed the reassembly of the most abundant HMW adiponectin species, the octadecamer, following its collapse to smaller oligomers in vitro. Purified bovine serum adiponectin octadecamer was treated with reducing agents at pH 5 to obtain trimers. These reduced trimers partially and spontaneously reassembled into octadecamers upon oxidative formation of disulfide bonds. Disulfide bonds appear to occupy a greater role in the process of oligomerization than in the structural stabilization of mature octadecamer. Stable octadecamers lacking virtually all disulfide bonds could be observed in abundance using native gel electrophoresis, dynamic light scattering, and collision-induced dissociation nanoelectrospray ionization mass spectrometry. These findings indicate that while disulfide bonds help to maintain ...Continue Reading

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Citations

Feb 22, 2012·The Journal of Biological Chemistry·Marcus M SeldinG William Wong
May 24, 2011·BMC Biochemistry·David B BriggsTsu-Shuen Tsao
Aug 31, 2013·Reviews in Endocrine & Metabolic Disorders·Tsu-Shuen Tsao
Aug 22, 2013·Reviews in Endocrine & Metabolic Disorders·Marcus M SeldinG William Wong
Jan 12, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·David B BriggsTsu-Shuen Tsao
Feb 27, 2013·Biochemical and Biophysical Research Communications·Luca VanellaNader G Abraham
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Jun 11, 2015·The Journal of Biological Chemistry·Lutz HampeAlok K Mitra
Jan 1, 2020·Antioxidants & Redox Signaling·Lars BellnerEllen A Thompson
Aug 20, 2019·Archives of Biochemistry and Biophysics·George S DrummondNader G Abraham
Apr 4, 2021·International Journal of Molecular Sciences·Damian NeubauerWojciech Kamysz

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