PMID: 8954155Nov 15, 1996Paper

Disulphide bonding in a stylar self-incompatibility ribonuclease of Nicotiana alata

European Journal of Biochemistry
D Oxley, A Bacic

Abstract

Many flowering plants have developed a self-incompatibility mechanism, which is controlled by a single polyallelic locus (the S-locus), to prevent inbreeding. The products of the S-locus in the styles of solanaceous plants are an allelic series of glycoproteins with RNase activity [McClure, B. A., Haring, V., Ebert, P. R., Anderson, M. A., Simpson, R. J., Sakiyama, F. & Clarke, A. E. (1989) Nature 342, 955-957]. These S-RNases show some amino-acid-sequence similarity with two fungal RNases (T2 and Rh), including the presence of two active-site His residues, which suggests a common three-dimensional structure. Disulphide bonding is important in the maintenance of the three-dimensional structure of the fungal RNases [Kurihara, H., Mitsui, Y., Ohgi, K., Irie, M., Mizuno, H. & Nakamura, T. (1992) FEBS Lett. 306, 189-192] and the S-RNases [Tsai, D. S., Lee, H.-S., Post, L. C., Kreiling, K. M. & Kao, T.-H. (1992) Sex. Plant Reprod. 5, 256-263]. We have used the S2-allele RNase of Nicotiana alata, which has nine Cys residues, to establish the pattern of disulphide bonding. The disulphide bonds Cys16-Cys21, Cys45-Cys94, Cys153-Cys182 and Cys165-Cys176 are consistent with the S2-RNase having a similar three-dimensional structure to RNas...Continue Reading

References

Mar 1, 1986·Analytical Biochemistry·R YazdanparastJ E Dixon
Mar 1, 1969·Archives of Biochemistry and Biophysics·W A SchroederJ R Shelton
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·D P MattonE Newbigin
May 1, 1977·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·K Sree RamuluM Devreux

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Citations

Dec 18, 2004·Plant Molecular Biology·Zhong ChenDaniel R Gallie
Dec 16, 2005·The Journal of Biological Chemistry·Javier Andrés Juárez-DíazFelipe Cruz-García
Jul 20, 2020·Journal of Structural Biology·María Daniela Torres-RodríguezFelipe Cruz-García
May 10, 2000·Journal of Molecular Biology·N TanakaK T Nakamura

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