Dithiothreitol improves recovery from in vitro diaphragm fatigue

Medicine and Science in Sports and Exercise
P T DiazT L Clanton

Abstract

There is increasing evidence that reactive oxygen species are produced during strenuous skeletal muscle work and that they contribute to the development of muscle fatigue. Although the precise cellular mechanisms underlying such a phenomenon remain obscure, it has been hypothesized that endogenously produced reactive oxygen species may down-regulate force production during fatigue by oxidizing critical sulfhydryl groups on important contractile proteins. To test this hypothesis, we fatigued rat diaphragm strips in vitro for 4 min at 20 Hz stimulation and a duty cycle of 0.33. Following fatigue, the tissue baths were drained and randomly replaced with either physiologic saline or physiologic saline containing the disulfide reducing agent, dithiothreitol (DTT) at varying doses (0.1-5.0 mM). Force-frequency characteristics were then measured over a 90-min recovery period. At the 0.5 and 1.0 mM doses, DTT treatment was associated with significantly greater force production in the recovery period. DTT's effects were observed at most frequencies tested, but appeared more prominent at the higher frequencies. The beneficial effects of DTT were not evident at the 0.1 or 5.0 mM doses and appeared to be specific for fatigued muscle. These...Continue Reading

References

Feb 1, 1992·Archives of Biochemistry and Biophysics·H XiongJ J Abramson
Feb 1, 1992·Journal of Applied Physiology·A AnzuetoS G Jenkinson
Aug 31, 1982·Biochemical and Biophysical Research Communications·K J DaviesL Packer
Dec 8, 1994·Nature·L KobzikJ S Stamler
Nov 1, 1994·Journal of Applied Physiology·P T DiazT L Clanton
Jan 1, 1995·Free Radical Biology & Medicine·W Vogt
Jul 1, 1994·Journal of Applied Physiology·F A Khawli, M B Reid
Dec 1, 1994·The Journal of Clinical Investigation·M B ReidA A Leis
Aug 1, 1994·Journal of Applied Physiology·G BorzoneT L Clanton
May 1, 1994·Journal of Applied Physiology·M B Reid, M R Moody
Apr 1, 1994·American Journal of Respiratory and Critical Care Medicine·A AnzuetoS G Jenkinson
Jan 1, 1993·The American Review of Respiratory Disease·E NashawatiG Supinski
Jan 1, 1993·Journal of Applied Physiology·A AnzuetoS G Jenkinson

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Citations

Jul 25, 2000·Acta Physiologica Scandinavica·J M Lawler, Z Hu
Nov 17, 2007·Journal of Applied Physiology·Leonardo F Ferreira, Michael B Reid
Sep 5, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·S Ryan OliverThomas L Clanton
Mar 8, 2011·Antioxidants & Redox Signaling·Håkan Westerblad, David G Allen
Jun 11, 1998·Journal of Applied Physiology·P MohanrajT L Clanton
May 2, 2009·Journal of Applied Physiology·Leonardo F FerreiraMichael B Reid
Oct 23, 2002·Journal of Applied Physiology·Toshiharu ObaMamoru Yamaguchi
Mar 1, 2013·Oral Diseases·C M Shortt, K D O'Halloran
Oct 17, 2008·Physiological Reviews·Scott K Powers, Malcolm J Jackson
Jan 15, 2008·Free Radical Biology & Medicine·Michael B Reid

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