Diurnal modulation of the Na+/K+-ATPase and spontaneous firing in the rat retinorecipient clock neurons

Journal of Neurophysiology
Han-Ying Wang, Rong-Chi Huang

Abstract

The ventral "core" suprachiasmatic nucleus (vSCN) neurons are the retinorecipient neurons in the mammalian circadian clock and maintain a diurnal firing rhythm in reduced preparations. We tested the possibility that daily changes in Na+/K+-ATPase accompany diurnal variation in spontaneous electrical activity. In control, bath application of 9 microM strophanthidin increased the spontaneous firing both at day and night but to different extents. In the presence of 1 mM Ni2+ to block spontaneous firing, addition of 9 microM strophanthidin, but not higher concentrations (6.5-20 mM) of external K+, induced the silenced cells to fire action potentials in a diurnal rhythm, suggesting a diurnal change in Na+/K+-ATPase activity. Consistently, voltage-clamp recordings demonstrated that the pump current blocked by 9 microM strophanthidin was approximately three times larger in daytime than nighttime and was little affected by the presence of 1 mM Ni2+. Experiments with various concentrations of strophanthidin further suggests day-night differences in maximum Na+/K+-ATPase activity, amounting to 6 pA of pump current at day and down to 3.5 pA at night, and in its half-block concentrations, changing from a daytime value of 4 microM to a nigh...Continue Reading

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Citations

Sep 3, 2011·Nature Reviews. Neuroscience·Christopher S Colwell
Aug 6, 2011·Cephalalgia : an International Journal of Headache·Michael G HarringtonXianghong Arakaki
Mar 9, 2010·Cerebrospinal Fluid Research·Michael G HarringtonNathan F Dalleska
Mar 4, 2009·The Journal of Physiology·Chun-Hao ChenRong-Chi Huang
May 12, 2009·Journal of Insect Physiology·Jolanta Górska-AndrzejakElzbieta Pyza
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Jan 9, 2015·Journal of Neurophysiology·Yi-Chi WangRong-Chi Huang
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Oct 3, 2019·International Journal of Molecular Sciences·Ruo-Ciao ChengRong-Chi Huang
May 18, 2018·Frontiers in Neural Circuits·Wojciech KrzeptowskiElżbieta Pyza

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