PMID: 6538567Apr 10, 1984Paper

Diurnal variation of cytosolic fatty acid-binding protein content and of palmitate oxidation in rat liver and heart.

The Journal of Biological Chemistry
J F GlatzH J Kempen

Abstract

Delipidated proteins from albumin-free liver and heart cytosol obtained from rats sacrificed at the mid-dark or the mid-light phase of the light cycle were assayed for their palmitate-binding capacity. In both tissues a marked variation of this binding capacity was observed from about 3-4 nmol/mg of protein in the mid-light phase of the cycle to about 7-8 nmol/mg of protein in the mid-dark phase. Sephadex G-75 chromatography of the cytosolic proteins revealed that the palmitate binding could in all cases almost entirely be attributed to proteins of Mr = 12,000-14,000, suggesting that the observed diurnal variations are related to differences in the content of fatty acid-binding protein (FABP). In both rat liver and heart FABP represents about 4 (mid-light) to 8% (mid-dark) of the total soluble proteins. Cholestyramine feeding increased the FABP content of liver cytosol from rats sacrificed at the mid-light phase, but not in those sacrificed at the mid-dark phase, in such a way that the diurnal variation of the FABP content virtually disappeared. The palmitate oxidation capacity and citrate synthase activity also exhibited a concomitant diurnal periodicity in rat liver and, to a lesser extent, in rat heart. The results provide a...Continue Reading

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