PMID: 9192167Jun 1, 1997Paper

Diurnal variations in tear glycoproteins: evidence for an epithelial origin for the major non-reducible > or = 450 kDa sialoglycoprotein(s)

Current Eye Research
R A SackG Lew

Abstract

To characterize the nature and origin of changes in tear glycoproteins accompanying eye closure. Reflex (R) and overnight closed (C) eye tears collected by capillary tubes were centrifuged with the resulting R pellets (primarily desquamated epithelial cells) and C pellets (primarily PMN and some epithelial cells) extracted in acidic PBS. Extracts and supernatants were separated by size-exclusion HPLC and/or SDS-PAGE. Gels were stained or blotted and immune- or lectin-probed. An HPLC glycoprotein fraction of > or = 450 kDa isolated from all four sources was characterized before and after partial deglycosylation, using antibodies specific to known mucin and carbohydrate epitopes. Immunofluorescence microscopy was carried out on human conjunctiva, using as probe a MAb to salivary mucin specific for a sialyl Lea epitope, which was found to cross-react specifically with the major non-reducible high molecular weight sialoglycoproteins (SGs) in tears. These SGs were immunoprecipitated and blot-probed along with tissue extracts. R fluid contained minor amounts of numerous glycoproteins, including probably several of inducible lacrimal secretory origin. Results confirmed sIgA as the principal source of the intense reducible glycoprotein...Continue Reading

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Citations

Sep 1, 2007·Proteomics. Clinical Applications·Kaili Wu, Yanli Zhang
Jul 16, 2005·Current Eye Research·Nancy A McNamaraSuzanne M J Fleiszig
May 10, 2008·Glycoconjugate Journal·Louise RoyleStephen D Carrington
Dec 22, 2010·Acta Medica (Hradec Králové)·Reyhaneh SaririReza Hassan Sajedi
Jul 15, 2005·Cornea·Scott A ReadLeo G Carney
Aug 5, 2008·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Shimin LiNancy A McNamara
Oct 13, 2000·Progress in Retinal and Eye Research·R A SackB Bogart

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