DIVA: Natural Navigation Inside 3D Images Using Virtual Reality.

Journal of Molecular Biology
Mohamed El BeheiryJean-Baptiste Masson

Abstract

As three-dimensional microscopy becomes commonplace in biological research, there is an increasing need for researchers to be able to view experimental image stacks in a natural three-dimensional viewing context. Through stereoscopy and motion tracking, commercial virtual reality headsets provide a solution to this important visualization challenge by allowing researchers to view volumetric objects in an entirely intuitive fashion. With this motivation, we present DIVA, a user-friendly software tool that automatically creates detailed three-dimensional reconstructions of raw experimental image stacks that are integrated in virtual reality. In DIVA's immersive virtual environment, users can view, manipulate and perform volumetric measurements on their microscopy images as they would to real physical objects. In contrast to similar solutions, our software provides high-quality volume rendering with native TIFF file compatibility. We benchmark the software with diverse image types including those generated by confocal, light-sheet and electron microscopy. DIVA is available at https://diva.pasteur.fr and will be regularly updated.

References

Jun 30, 2012·Nature Methods·Fabrice de ChaumontJean-Christophe Olivo-Marin
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Jul 12, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gerald J SunGuo-li Ming
Sep 4, 2017·IEEE Transactions on Visualization and Computer Graphics·Will UsherValerio Pascucci
Nov 22, 2017·Traffic·Angus P R JohnstonRobert G Parton
Jun 28, 2018·Journal of Molecular Biology·Caroline StefaniThomas Skillman
Feb 10, 2019·Journal of Molecular Biology·Mohamed El BeheiryJean-Baptiste Masson

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Citations

Mar 25, 2021·Journal of Cardiac Surgery·Francesca RaimondiJean-Baptiste Masson
Oct 3, 2020·Journal of Stomatology, Oral and Maxillofacial Surgery·Jebrane BouaoudRoman H Khonsari
Nov 13, 2021·JCO Clinical Cancer Informatics·Mohamed El BeheiryCaroline Malhaire

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