Divalent cation distribution in dinoflagellate chromosomes imaged by high-resolution ion probe mass spectrometry

European Journal of Cell Biology
R Levi-SettiP J Rizzo

Abstract

From a variety of analytical electron microscopy experiments, the chromosomes of dinoflagellates are known to contain sizeable amounts of cations, the latter thought to contribute to the neutralization of the negative charge carried by the phosphate groups in the DNA backbone. From previous Ca and Mg chelation experiments, it is also known that these cations are necessary for the compaction and preservation of the chromosome architecture. Similar conclusions have been recently presented by our group concerning mammalian mitotic chromosomes, in studies based on secondary ion mass spectrometry (SIMS) carried out with the University of Chicago high-resolution scanning ion microprobe (UC-SIM). We have now applied this instrument to image the distribution of DNA-bound Ca(2+) and Mg(2+) in dinoflagellate chromosomes, a goal that could not be attained earlier by analytical electron microscopy. Analyzed quantitatively and imaged here by SIMS for the first time, through their cation content, are the chromosomes of the dinoflagellates Prorocentrum micans, Gymnodinium mikimotoi and Gymnodinium dorsum. The cell nuclei were isolated and prepared for SIMS analysis with a minimal protocol (mechanical fractionation in culture medium followed b...Continue Reading

References

Jan 1, 1975·Hereditas·O K Haapala, M O Soyer
Aug 1, 1990·The Journal of Cell Biology·M O Soyer-GobillardE Delain
Jan 1, 1988·Annual Review of Biophysics and Biophysical Chemistry·R Levi-Setti
Aug 15, 1973·Nature: New Biology·O K Haapala, M O Soyer
Jan 1, 1981·Protoplasma·D L SpectorR E Triemer
Nov 1, 1994·Biopolymers·E R Arndt, E S Stevens
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·I KoltoverC R Safinya
Sep 17, 2003·Cell Research·Peter J Rizzo

❮ Previous
Next ❯

Citations

Sep 24, 2009·Chemical Reviews·Reagan McRaeChristoph J Fahrni
Oct 18, 2012·DNA and Cell Biology·Shiyong SunFaqin Dong
Dec 22, 2012·Current Biology : CB·Paul B Talbert, Steven Henikoff
Apr 9, 2018·Biochemistry. Biokhimii︠a︡·T CremerC Cremer
Apr 19, 2018·Metallomics : Integrated Biometal Science·Stefan K KolevGeorgi N Vayssilov
Apr 18, 2019·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Astari DwirantiKiichi Fukui
Aug 17, 2019·Microorganisms·Bahareh ZaheriDavid Morse
Aug 11, 2019·Microorganisms·Sebastian G GornikRoss F Waller
Jan 26, 2017·Harmful Algae·Kaytee L PokrzywinskiKathryn J Coyne

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.