PMID: 42003Jan 1, 1979

Divalent cation-induced aggregation of chromaffin granule membranes

Membrane Biochemistry
S J MorrisD H Haynes

Abstract

Divalent cations induce the aggregation of chromaffin granule ghosts (CG membranes) at millimolar concentrations. Monovalent cations produce the same effect at 100-fold higher concentrations. The kinetics of the dimerization phase were followed by light-scattering changes observed in stopped-flow rapid mixing experiments. The rate constant for Ca2+-induced dimerization (kapp) is 0.86-1.0 x 10(9) M-1sec-1, based on the "molar" vesicle concentration. This value is close to the values predicted by theory for the case of diffusion-controlled reaction (7.02 x 10(9) M-1sec-1), indicating that there is no energy barrier to dimerization. Arrhenius plots between 10 degrees and 42 degrees C support this; the activation energy observed, +4.4 Kcal, is close to the value (4.6-4.8 Kcal) predicted for diffusion control according to theory. Artificial vesicles prepared from CG lipids were also found to have cation-induced aggregation, but the rates (values of kapp) were less than 1/100 as large as those with native CG membranes. Also, significant differences were found with respect to cation specificity. It is concluded that the slow rates are due to the low probability that the segments of membrane which approach will be matched in polar head...Continue Reading

References

Apr 1, 1976·Journal of Ultrastructure Research·P A EaglesC Van Horn
Jan 14, 1976·Biochimica Et Biophysica Acta·J Hochman, R L Perlman
Jul 3, 1975·Biochimica Et Biophysica Acta·J Lansman, D H Haynes
Oct 17, 1975·Biochimica Et Biophysica Acta·W R RedwoodB C Patel
Oct 1, 1975·Journal of Theoretical Biology·P M Dean, E K Matthews
Jul 31, 1974·Biochimica Et Biophysica Acta·W EdwardsS J Morris
Sep 1, 1974·The Biochemical Journal·P M Dean, E K Matthews
Nov 1, 1974·The Biochemical Journal·J H Phillips
Jan 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H Träuble, H Eibl
May 30, 1974·Biochimica Et Biophysica Acta·D PapahadjopoulosW J Vail

Citations

Dec 1, 1986·Journal of Bioenergetics and Biomembranes·C Y Kwan
Jun 29, 1979·The Journal of Membrane Biology·M A Kolber, D H Haynes
Sep 30, 1980·The Journal of Membrane Biology·V C ChiuD H Haynes
Jan 1, 1983·Journal of the Autonomic Nervous System·S J MorrisD H Haynes
Jan 1, 1980·Neuroscience·H Winkler, E Westhead
Dec 21, 1979·Journal of Theoretical Biology·D H HaynesS J Morris
Nov 14, 1979·Biochemical and Biophysical Research Communications·S J Morris, J M Hughes
Sep 1, 1982·Metabolism: Clinical and Experimental·K E SussmanP S Mehler

Related Concepts

Adrenal Medulla
Calcium
Bos indicus
Chromaffin Granule
Chromaffin System
Hydrogen-Ion Concentration
Intracellular Membranes
Magnesium
Membrane Lipids

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