Divalent Cations Regulate the Ion Conductance Properties of Diverse Classes of Aquaporins

International Journal of Molecular Sciences
Mohamad KourghiAndrea J Yool

Abstract

Aquaporins (AQPs) are known to facilitate water and solute fluxes across barrier membranes. An increasing number of AQPs are being found to serve as ion channels. Ion and water permeability of selected plant and animal AQPs (plant Arabidopsis thaliana AtPIP2;1, AtPIP2;2, AtPIP2;7, human Homo sapiens HsAQP1, rat Rattus norvegicus RnAQP4, RnAQP5, and fly Drosophilamelanogaster DmBIB) were expressed in Xenopus oocytes and examined in chelator-buffered salines to evaluate the effects of divalent cations (Ca(2+), Mg(2+), Ba(2+) and Cd(2+)) on ionic conductances. AtPIP2;1, AtPIP2;2, HsAQP1 and DmBIB expressing oocytes had ionic conductances, and showed differential sensitivity to block by external Ca(2+). The order of potency of inhibition by Ca(2+) was AtPIP2;2 > AtPIP2;1 > DmBIB > HsAQP1. Blockage of the AQP cation channels by Ba(2+) and Cd(2+) caused voltage-sensitive outward rectification. The channels with the highest sensitivity to Ca(2+) (AtPIP2;1 and AtPIP2;2) showed a distinctive relief of the Ca(2+) block by co-application of excess Ba(2+), suggesting that divalent ions act at the same site. Recognizing the regulatory role of divalent cations may enable the discovery of other classes of AQP ion channels, and facilitate the ...Continue Reading

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Citations

Nov 15, 2018·Cells·Marcelo OzuGabriela Amodeo
Apr 21, 2019·The New Phytologist·Rana MunnsStephen D Tyerman
Aug 12, 2020·Biochemical Society Transactions·Maria HrmovaStephen D Tyerman
Jul 18, 2020·Plant, Cell & Environment·Jiaen QiuCaitlin S Byrt
Sep 11, 2020·Physiologia Plantarum·Gloria BarzanaMicaela Carvajal
Oct 1, 2020·International Journal of Molecular Sciences·Sen Thi Huong TranMaki Katsuhara
Feb 6, 2020·Frontiers in Cellular and Infection Microbiology·Patricia BarreraVeronica Jimenez
May 7, 2020·Frontiers in Plant Science·Lei Ding, François Chaumont
Dec 17, 2020·International Journal of Molecular Sciences·Yan WangFushun Hao
Mar 30, 2021·Plant Science : an International Journal of Experimental Plant Biology·Victoria VitaliGabriela Amodeo
Jun 1, 2021·Biochimica Et Biophysica Acta. Biomembranes·Samantha A McGaugheyCaitlin S Byrt
Feb 13, 2021·Annual Review of Plant Biology·Stephen D TyermanCaitlin S Byrt

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Datasets Mentioned

BETA
AAA66221.1

Methods Mentioned

BETA
confocal microscopy
dissecting

Software Mentioned

GraphPad
Prism
Clampex
Image J
pClamp

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