Divergence involving global regulatory gene mutations in an Escherichia coli population evolving under phosphate limitation.

Genome Biology and Evolution
Lei WangThomas Ferenci

Abstract

Many of the important changes in evolution are regulatory in nature. Sequenced bacterial genomes point to flexibility in regulatory circuits but we do not know how regulation is remodeled in evolving bacteria. Here, we study the regulatory changes that emerge in populations evolving under controlled conditions during experimental evolution of Escherichia coli in a phosphate-limited chemostat culture. Genomes were sequenced from five clones with different combinations of phenotypic properties that coexisted in a population after 37 days. Each of the distinct isolates contained a different mutation in 1 of 3 highly pleiotropic regulatory genes (hfq, spoT, or rpoS). The mutations resulted in dissimilar proteomic changes, consistent with the documented effects of hfq, spoT, and rpoS mutations. The different mutations do share a common benefit, however, in that the mutations each redirect cellular resources away from stress responses that are redundant in a constant selection environment. The hfq mutation lowers several individual stress responses as well the small RNA-dependent activation of rpoS translation and hence general stress resistance. The spoT mutation reduces ppGpp levels, decreasing the stringent response as well as rpo...Continue Reading

References

Aug 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Torriani
Jul 17, 1998·Nature·P B Rainey, M Travisano
May 14, 1999·Current Opinion in Microbiology·T Ferenci
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·D YuD L Court
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·M BarrierM D Purugganan
Nov 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·G M Edelman, J A Gally
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Regine Hengge-Aronis
Jul 2, 2004·Nature·Patricia J WittkoppAndrew G Clark
Aug 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·Shaobin ZhongAntony M Dean
Aug 20, 2004·Journal of Bacteriology·Thea KingThomas Ferenci
Sep 7, 2004·Microbiology·Natalia Pasternak TaschnerBeny Spira
Oct 19, 2004·Annual Review of Microbiology·Susan Gottesman
May 4, 2005·Trends in Microbiology·Lisa U MagnussonThomas Nyström
Apr 8, 2006·Genes & Development·Alexandre BougdourSusan Gottesman
Apr 28, 2006·Evolution; International Journal of Organic Evolution·Rowan D H Barrett, Graham Bell
Jul 11, 2006·Science·Ram MaharjanThomas Ferenci
Jul 15, 2006·Nucleic Acids Research·Irma Lozada-ChávezJulio Collado-Vides
Jan 24, 2007·PLoS Biology·Richard E LenskiCharles Ofria
Mar 21, 2007·Current Opinion in Microbiology·Poul Valentin-HansenAnders A Rasmussen
Mar 27, 2007·Current Opinion in Microbiology·Carin K Vanderpool
Aug 11, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Nadège PhilippeDominique Schneider
Aug 21, 2007·Advances in Microbial Physiology·Thomas Ferenci
Aug 24, 2007·Journal of Evolutionary Biology·I GudeljR C MacLean
Nov 28, 2007·Journal of Bacteriology·Tim DurfeeDing Jun Jin
May 6, 2008·Annual Review of Microbiology·Katarzyna Potrykus, Michael Cashel
Oct 1, 2008·Journal of Evolutionary Biology·B M FitzpatrickS Gavrilets
Oct 28, 2008·Applied and Environmental Microbiology·Vasiliy A PortnoyBernhard Ø Palsson
Nov 21, 2008·Ecology Letters·Daniel E RozenDominique Schneider

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Citations

Mar 15, 2011·Journal of Molecular Evolution·Joël GafféDominique Schneider
Apr 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Søren DamkiærLars Jelsbak
Dec 21, 2012·Genome Biology and Evolution·Nadia JezequelPhilippe Thomen
Dec 12, 2012·Biology Letters·Ram P MaharjanThomas Ferenci
Jul 14, 2011·Genome Research·Sung Ho YoonNitin S Baliga
Oct 16, 2012·Applied and Environmental Microbiology·Gonzalo N BidartPablo I Nikel
Dec 7, 2010·BMC Evolutionary Biology·Celeste J BrownAmber D Stancik
Jul 3, 2013·Microbial Cell Factories·Martin Dragosits, Diethard Mattanovich
Jun 12, 2012·Genome Biology·Ram P MaharjanLei Wang
May 28, 2014·Archives of Microbiology·Heloisa F GalbiatiBeny Spira
Aug 8, 2014·FEMS Microbiology Reviews·David Gresham, Jungeui Hong
Feb 21, 2016·Microbial Cell Factories·Patrice L Moreau, Laurent Loiseau
Oct 5, 2014·Genomics·David Gresham, Maitreya J Dunham
Jan 23, 2016·Archives of Microbiology·Fernanda Nogales da Costa VasconcelosBeny Spira
Dec 27, 2015·Trends in Microbiology·Thomas Ferenci
Jul 8, 2011·Molecular Systems Biology·Tom M ConradBernhard Ø Palsson
Feb 16, 2012·Molecular Ecology·Jeremy R DettmanRees Kassen
Aug 2, 2013·Ecology Letters·Ram MaharjanIvana Gudelj
Sep 17, 2013·Evolution; International Journal of Organic Evolution·Ram Prasad Maharjan, Thomas Ferenci
Mar 14, 2014·Molecular Ecology·Mickael Le GacDominique Schneider
Jun 28, 2015·International Journal of Food Microbiology·Avelino Alvarez-OrdóñezMiguel Prieto
Apr 11, 2013·Current Opinion in Biotechnology·Karl KochanowskiVictor Chubukov
Mar 21, 2015·Interdisciplinary Sciences, Computational Life Sciences·Li LiDong-Qing Wei
Jan 16, 2015·Genome Biology and Evolution·Ram P MaharjanLei Wang
Oct 22, 2017·Applied and Environmental Microbiology·Michelle Qiu CarterVivek Kapur
Apr 26, 2017·Journal of Bacteriology·Dagmara I KisielaEvgeni V Sokurenko
Nov 14, 2012·Nature Reviews. Microbiology·Anders FolkessonSøren Molin
Mar 28, 2012·Nature Reviews. Microbiology·Thomas HindréDominique Schneider

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