PMID: 9555879Apr 29, 1998Paper

Divergence of a DNA replication gene cluster in the T4-related bacteriophage RB69

Journal of Bacteriology
L S YehJ D Karam

Abstract

The genomes of bacteriophages T4 and RB69 are phylogenetically related but diverge in nucleotide sequence at many loci and are incompatible with each other in vivo. We describe here the biological implications of divergence in a genomic segment that encodes four essential DNA replication proteins: gp45 (sliding clamp), gp44/62 complex (clamp loader), and gp46 (a recombination protein). We have cloned, sequenced, and expressed several overlapping segments of the RB69 gene 46-45.2-(rpbA)-45-44-62 cluster and compared its features to those of the homologous gene cluster from T4. The deduced primary structures of all four RB69 replication proteins and gp45.2 from this cluster are very similar (80 to 95% similarity) to those of their respective T4 homologs. In contrast, the rpbA region (which encodes a nonessential protein in T4) is highly diverged (approximately 49% similarity) between the two phage genomes and does not encode protein in RB69. Expression studies and patterns of high divergence of intercistronic nucleotide sequences of this cluster suggest that T4 and RB69 evolved similar transcriptional and translational control strategies for the cistrons contained therein, but with different specificities. In plasmid-phage comple...Continue Reading

References

Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C E Jozwik, E S Miller
Jun 1, 1991·Genetics·M D Andrake, J D Karam
Jun 20, 1990·Journal of Molecular Biology·C TuerkL Gold
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M UzanE Brody
Jan 20, 1988·Journal of Molecular Biology·N GuildL Gold
Feb 1, 1987·Journal of Virology·K P WilliamsE P Geiduschek
Aug 10, 1984·Nucleic Acids Research·M TrojanowskaL Gold
Jan 1, 1995·Annual Review of Biochemistry·Z Kelman, M O'Donnell
Nov 3, 1995·The Journal of Biological Chemistry·C C WangJ D Karam
Sep 9, 1994·Cell·B Stillman
Mar 28, 1997·Journal of Molecular Biology·C MonodH M Krisch
Jul 11, 1997·The Journal of Biological Chemistry·C C WangJ D Karam
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·H K DressmanJ W Drake

❮ Previous
Next ❯

Citations

Dec 10, 1999·The Journal of Biological Chemistry·D M JanzenM K Reddy
Dec 15, 2000·Journal of Bacteriology·F TétartH M Krisch
Oct 19, 2004·Journal of Bacteriology·Sandra Chibani-ChennoufiHarald Brüssow
Jan 13, 2001·The Journal of Biological Chemistry·A BebenekJ W Drake
Apr 27, 2002·Journal of Bacteriology·Carine DesplatsH M Krisch
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·G J LathamP H von Hippel
Apr 7, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Thomas A Steitz, Y Whitney Yin
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Eric S MillerWolfgang Rüger
Dec 4, 2004·Journal of Bacteriology·Sandra Chibani-ChennoufiHarald Brüssow
Nov 21, 2012·Journal of Virology·Fang TangChengping Lu
Jun 28, 2002·The Journal of Biological Chemistry·Vasiliy M PetrovJim D Karam
Mar 4, 2000·Journal of Molecular Biology·I MoarefiJ Kuriyan
Jun 12, 2003·Research in Microbiology·Carine Desplats, Henry M Krisch
May 4, 2005·Virology·Timothy R DeanEric S Miller
Jul 11, 2006·Journal of Molecular Biology·Vasiliy M PetrovJim D Karam
Oct 30, 2010·Virology Journal·Vasiliy M PetrovJim D Karam
Nov 10, 2009·Journal of Molecular Biology·Vasiliy M PetrovJim D Karam

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.