DOI: 10.1101/463950Dec 6, 2018Paper

Divergent effects of acute and prolonged interleukin 33 exposure onmast cell IgE-mediated functions

BioRxiv : the Preprint Server for Biology
E. RönnbergGunnar Nilsson

Abstract

BackgroundEpithelial cytokines, including IL-33 and TSLP, have attracted interest because of their roles in chronic allergic inflammation-related conditions such as asthma. Mast cells are one of the major targets of IL-33, to which they respond by secreting cytokines. Most studies performed thus far have investigated the acute effects of IL-33 on mast cells.\n\nObjectiveThe objective of this study is to investigate how acute versus prolonged exposure of human mast cells to IL-33 and TSLP affects mediator synthesis and IgE-mediated activation.\n\nMethodsHuman lung mast cells (HLMCs), cord blood-derived mast cells (CBMCs), and the ROSA mast cell line were used for this study. Surface receptor expression and the levels of mediators were measured after treatment with IL-33 and/or TSLP.\n\nResultsIL-33 induced the acute release of cytokines. Prolonged exposure to IL-33 increased while TSLP reduced intracellular levels of tryptase. Acute IL-33 treatment strongly potentiated IgE-mediated activation. In contrast, four days of exposure to IL-33 decreased IgE-mediated activation, an effect that was accompanied by a reduction in Fc{varepsilon}RI expression.\n\nConclusion & Clinical RelevanceWe show that IL-33 plays dual roles for mast cel...Continue Reading

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Methods Mentioned

BETA
flow cytometry
FCS
ELISA

Software Mentioned

FlowJo
GraphPad Prism

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