Divergent effects of corticotropin releasing hormone on endothelial cell nitric oxide synthase are associated with different expression of CRH type 1 and 2 receptors

British Journal of Pharmacology
G CantarellaR Bernardini

Abstract

1. Endothelium is a target for an array of factors involved in inflammation. Endothelial cells express receptors for CRH, a neuropeptide produced during inflammation. We report both the concentration-dependent inhibitory effect of CRH upon cytokine-stimulated nitrite release by H5V murine endothelioma cells, and its stimulatory one in HUVEC cells. 2. Western blot analysis showed that CRH inhibits cytokine-stimulated iNOS protein in H5V cells, and, instead, potentiated it in HUVEC cells. 3. H5V cells expressed both CRH receptors (CRH-R1 and R2) mRNAs, whereas HUVEC cells expressed the CRH-R2 mRNA solely. 4. CRH increased medium nitrites and iNOS protein expression in H5V cells pretreated with the selective CRH-R1 antagonist CP 154,526. However, the selective CRH-R2 antagonist anti-Svg-30 failed to produce similar effects. In fact, anti-Svg-30 inhibited CRH-induced increase of nitrite release and iNOS expression in HUVEC cells. 5. Our results confirm the activating role of CRH on endothelial cells, although it suggests its possible inhibitory role in the late phase of the inflammatory response. NO-mediated effects of CRH on endothelial cells could be exploited in therapeutic strategies related to inflammatory and/or degenerative ...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S LamasT Michel
Apr 15, 1991·Cellular Immunology·T AudhyaC S Hollander
Oct 1, 1991·The Journal of Immunology : Official Journal of the American Association of Immunologists·F BussolinoA Mantovani
Mar 1, 1990·Brain, Behavior, and Immunity·A StephanouS L Lightman
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R ChenW W Vale
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·M PerrinW Vale
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·D W SchulzJ Heym
Jan 14, 1997·European Journal of Pharmacology·S G CorreaI D Bianco
Apr 1, 1997·The Journal of Clinical Endocrinology and Metabolism·Y SakaiT Suda
Jul 22, 1998·European Journal of Pharmacology·S IavicoliT Kishimoto
Aug 12, 1999·The Journal of Clinical Endocrinology and Metabolism·T SimonciniF Petraglia
Mar 11, 2000·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·K M Rao

❮ Previous
Next ❯

Citations

Feb 7, 2002·Trends in Pharmacological Sciences·Frank M Dautzenberg, Richard L Hauger
Nov 15, 2002·Trends in Endocrinology and Metabolism : TEM·Dimitris K Grammatopoulos, George P Chrousos
Mar 25, 2009·Pharmacological Reports : PR·Sean M Davidson, Derek M Yellon
Oct 16, 2012·Gastroenterology·Bruno L Bonaz, Charles N Bernstein
Sep 3, 2011·British Journal of Pharmacology·Dimitris K Grammatopoulos
Mar 10, 2015·Drug Discovery Today·Rui AdãoCarmen Brás-Silva
Jul 24, 2004·Molecular Pharmacology·Pieter J PeetersThomas Steckler
Jul 23, 2020·Trends in psychiatry and psychotherapy·Mailton VasconcelosRosa Maria M de Almeida
Feb 22, 2003·CNS Drug Reviews·Patricia A SeymourDavid W Schulz
Oct 17, 2019·Journal of Clinical Medicine·Stavroula BaritakiCharalabos Pothoulakis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.