PMID: 9163353Apr 15, 1997Paper

Divergent effects of extracellular and intracellular alkalosis on Ca2+ entry pathways in vascular endothelial cells

The Biochemical Journal
I Wakabayashi, K Groschner

Abstract

Modulation by alkalosis of basal leak Ca2+ entry and store-depletion-induced Ca2+ entry was investigated in the vascular endothelial cell line ECV 304. Ca2+ entry was monitored as the increase in the intracellular free Ca2+ concentration ([Ca2+]i) induced by elevation of the extracellular Ca2+ concentration. When ECV 304 cells were challenged with 100 nM thapsigargin in nominally Ca2+-free solution, [Ca2+]i increased transiently, and the increase in [Ca2+]i during a subsequent cumulative elevation of extracellular Ca2+ (from nominally Ca2+-free up to 5 mM) was markedly enhanced compared with non-stimulated cells (i.e. basal Ca2+ leak). Prolonged elevation of the extracellular pH (pHo) from 7.4 to 7.9 did not affect resting [Ca2+]i or the thapsigargin-induced [Ca2+]i transient evoked in nominally Ca2+-free solution, but increased leak Ca2+ entry as well as store-depletion-activated Ca2+ entry significantly. Basal Ca2+ leak and store-depletion-activated Ca2+ entry were enhanced either by acute elevation of pHo from 7.4 to 7.9 or by chronic alkalosis (pHo=7.9). Stimulation of Ca2+ entry by extracellular alkalosis was observed both in normal and in high extracellular K+ (110 mM) solution, suggesting that the effects of alkalosis ar...Continue Reading

Citations

Apr 5, 2012·Experimental Diabetes Research·Daniel R Beriault, Geoff H Werstuck
Jul 30, 2011·Journal of Critical Care·Ettore CrimiClaudio Napoli
Dec 1, 2004·The Journal of Biological Chemistry·Krzysztof ZabłockiJerzy Duszyński
Aug 19, 2010·The Journal of Membrane Biology·Matthieu MarinJean-Pierre Vilain
Oct 11, 2005·Apoptosis : an International Journal on Programmed Cell Death·M CutaiaG S Sidhu
Oct 1, 2010·American Journal of Physiology. Cell Physiology·Kurt A SchalperJuan C Sáez
May 31, 2003·Journal of Cardiovascular Pharmacology·Ichiro WakabayashiYoko Sotoda
Feb 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·M A Vander HeydenJ B Gordon
Jan 28, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·John B GordonJane A Madden
Apr 27, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·T R HallaJ B Gordon
Jun 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shiro MizunoTakeshi Ishizaki

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