Divergent effects of repeated cocaine and novel environment exposure on locus coeruleus c-fos expression and brain catecholamine concentrations in rats

Brain and Behavior
Michael J LisieskiShane A Perrine

Abstract

Chronic administration of cocaine causes a disinhibited, hyperexploratory response to novel environments. As the norepinephrine (NE) system regulates exploration and is dysregulated following cocaine exposure, we hypothesized that this cocaine-mediated hyperexploratory response is associated with increased locus coeruleus (LC) reactivity. To test this hypothesis, we used dual fluorescent in situ hybridization immunofluorescence to analyze novelty-induced c-fos and tyrosine hydroxylase expression in the LC and high-pressure liquid chromatography to measure dopamine (DA) and NE concentrations in key catecholamine projection regions following exposure to cocaine. Repeated cocaine exposure followed by a 14-day drug-free period increased exploration of novel environments, replicating previous findings. Novelty exposure increased LC c-fos expression, increased anterior cingulate NE, and decreased ventral tegmental area DA. Cocaine exposure decreased amygdala (AMY) DA, but had no effect on LC c-fos expression or NE in any tested brain region. No interactions between cocaine and novelty were found. Open arm exploration was positively correlated with LC c-fos expression and NE concentrations in both the anterior cingulate and nucleus ac...Continue Reading

References

Feb 3, 2004·The American Journal of Psychiatry·Clinton D KiltsKaren P G Drexler
Mar 15, 2005·Journal of Neuroscience Methods·Deborah C MashJohn Pablo
Jul 19, 2005·Annual Review of Neuroscience·Gary Aston-Jones, Jonathan D Cohen
Oct 29, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Heinrich S GompfJun Lu
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Sep 5, 2014·Journal of Addiction Medicine·Stéphane PotvinJulie Pelletier
Nov 27, 2014·Expert Opinion on Emerging Drugs·Daryl ShorterThomas R Kosten
Feb 28, 2015·Behavioural Brain Research·Samantha M KellerDayan Knox
Sep 29, 2015·Current Neuropharmacology·Magdalena ZaniewskaEdmund Przegalinski
May 7, 2016·Nature Reviews. Neuroscience·Marina E Wolf
Nov 17, 2017·Nature Reviews. Neuroscience·Nora D VolkowRuben Baler

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