Divergent Evolution of Eukaryotic CC- and A-Adding Enzymes

International Journal of Molecular Sciences
Lieselotte ErberMario Mörl

Abstract

Synthesis of the CCA end of essential tRNAs is performed either by CCA-adding enzymes or as a collaboration between enzymes restricted to CC- and A-incorporation. While the occurrence of such tRNA nucleotidyltransferases with partial activities seemed to be restricted to Bacteria, the first example of such split CCA-adding activities was reported in Schizosaccharomycespombe. Here, we demonstrate that the choanoflagellate Salpingoecarosetta also carries CC- and A-adding enzymes. However, these enzymes have distinct evolutionary origins. Furthermore, the restricted activity of the eukaryotic CC-adding enzymes has evolved in a different way compared to their bacterial counterparts. Yet, the molecular basis is very similar, as highly conserved positions within a catalytically important flexible loop region are missing in the CC-adding enzymes. For both the CC-adding enzymes from S.rosetta as well as S. pombe, introduction of the loop elements from closely related enzymes with full activity was able to restore CCA-addition, corroborating the significance of this loop in the evolution of bacterial as well as eukaryotic tRNA nucleotidyltransferases. Our data demonstrate that partial CC- and A-adding activities in Bacteria and Eukaryot...Continue Reading

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Citations

Jul 29, 2020·International Journal of Molecular Sciences·Lieselotte ErberMario Mörl
Dec 3, 2020·International Journal of Molecular Sciences·Oliver HennigMario Mörl

Methods Mentioned

BETA
in vitro transcription

Related Concepts

Transfer RNA
TRNA adenylyltransferase
Schizosaccharomyces pombe
Loop
Aptamer
TRNA Nucleotidyltransferase Activity
TRNA 3'-terminal CCA Addition
Asphondylia rosetta
TRNT1

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