Divergent invariant natural killer T-cell response to sepsis of abdominal vs. non-abdominal origin in human beings.

Surgical Infections
John S YoungDaithi S Heffernan

Abstract

The etiology of sepsis is broad. The peritoneal cavity displays compartmentalization with respect to inflammatory responses, so peripheral blood responses to sepsis of abdominal vs. non-abdominal origin are expected to be divergent. Lymphocytes and invariant natural killer T (iNKT) cells play important roles in survival from sepsis, as they dampen the neutrophil and macrophage responses. We assessed whether circulating iNKT cells display distinct phenotypic profiles depending on the presence of abdominal vs. non-abdominal infection with sepsis. Patients with sepsis, defined as infection confirmed microbiologically with a systemic inflammatory response syndrome (SIRS), were enrolled prospectively. They were categorized as having either exclusively sepsis of abdominal or exclusively non-abdominal origin. The white blood cell (WBC) count was recorded. Whole-blood staining with monoclonal antibodies to CD3, V-alpha-24 (to identify iNKT cells), and CD69 (marker of early activation) was applied. Of the 53 enrolled patients, 18 had abdominal infection. Pneumonia was the most common non-abdominal type. There was no difference in gender, age, Acute Physiology and Chronic Health Evaluation (APACHE) II score, WBC count, or CD3(+) T cells ...Continue Reading

References

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Citations

Apr 18, 2016·La Presse médicale·Kevin W McConnell, Craig M Coopersmith
Nov 1, 2016·Irish Journal of Medical Science·D S HeffernanAlfred Ayala

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Methods Mentioned

BETA
blood draws
bronchoalveolar lavage
blood draw
fluorescence-activated cell sorting

Software Mentioned

SigmaPlot

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