Divergent SATB1 expression across human life span and tissue compartments

Immunology and Cell Biology
Simone NüssingKatherine Kedzierska

Abstract

Special AT-rich binding protein-1 (SATB1) is a global chromatin organizer capable of activating or repressing gene transcription in mice and humans. The role of SATB1 is pivotal for T-cell development, with SATB1-knockout mice being neonatally lethal, although the exact mechanism is unknown. Moreover, SATB1 is dysregulated in T-cell lymphoma and proposed to suppress transcription of the Pdcd1 gene, encoding the immune checkpoint programmed cell death protein 1 (PD-1). Thus, SATB1 expression in T-cell subsets across different tissue compartments in humans is of potential importance for targeting PD-1. Here, we comprehensively analyzed SATB1 expression across different human tissues and immune compartments by flow cytometry and correlated this with PD-1 expression. We investigated SATB1 protein levels in pediatric and adult donors and assessed expression dynamics of this chromatin organizer across different immune cell subsets in human organs, as well as in antigen-specific T cells directed against acute and chronic viral infections. Our data demonstrate that SATB1 expression in humans is the highest in T-cell progenitors in the thymus, and then becomes downregulated in mature T cells in the periphery. Importantly, SATB1 expressi...Continue Reading

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Citations

Aug 28, 2019·International Journal of Molecular Sciences·Natalia Glatzel-PlucińskaMarzenna Podhorska-Okołów
Dec 23, 2020·Molecular Immunology·Marion SalouOlivier Lantz
Dec 29, 2020·Signal Transduction and Targeted Therapy·Hong-Yi ZhengYong-Tang Zheng
Dec 30, 2020·International Journal of Molecular Sciences·Taylah J BennettStephen J Turner
Jun 1, 2021·Frontiers in Immunology·Miguel Ángel Palacios-PedreroGiulietta Saletti
Feb 23, 2021·Genome Génome / Conseil National De Recherches Canada·Delong FengBingtao Hao
Sep 5, 2021·BMC Medical Genomics·Timothy A McCaffreyGeorges St Laurent

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Methods Mentioned

BETA
flow cytometry
FACS
FCS

Software Mentioned

FlowJo
GraphPad
DonateLife
GraphPad Prism

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