Divergent transcriptional control of multidrug resistance genes in Saccharomyces cerevisiae.

The Journal of Biological Chemistry
T C Hallstrom, W Scott Moye-Rowley

Abstract

Improper control of expression of ATP binding cassette transporter-encoding genes is an important contributor to acquisition of multidrug resistance in human tumor cells. In this study, we have analyzed the function of the promoter region of the Saccharomyces cerevisiae YOR1 gene, which encodes an ATP binding cassette transporter protein that is required for multidrug tolerance in S. cerevisiae. Deletion analysis of a YOR1-lacZ fusion gene defines three important transcriptional regulatory elements. Two of these elements serve to positively regulate expression of YOR1, and the third element is a negative regulatory site. One positive element corresponds to a Pdr1p/Pdr3p response element, a site required for transcriptional control by the homologous zinc finger transcription factors Pdr1p and Pdr3p in other promoters. The second positive element is located between nucleotides -535 and -299 and is referred to as UASYOR1 (where UAS is upstream activation sequence). Interestingly, function of UASYOR1 is inhibited by the downstream negative regulatory site. Promoter fusions constructed between UASYOR1 and the PDR5 promoter, another gene under Pdr1p/Pdr3p control, are active, whereas analogous promoter fusions constructed with the CY...Continue Reading

References

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Citations

Aug 11, 2004·Eukaryotic Cell·Marcin KolaczkowskiW Scott Moye-Rowley
Jul 16, 2011·Journal of Molecular Microbiology and Biotechnology·Luca Riccardo Formenti, Morten C Kielland-Brandt
Jan 5, 2007·Biological & Pharmaceutical Bulletin·Hui ShenYuan-Ying Jiang
May 18, 2005·Biochemical and Biophysical Research Communications·Naseem Akhtar GaurRajendra Prasad
May 5, 2004·The Journal of Biological Chemistry·Bassel AkacheBernard Turcotte
Apr 27, 2001·The Journal of Biological Chemistry·T C HallstromW S Moye-Rowley
May 16, 2000·The Journal of Biological Chemistry·X J ChenG D Clark-Walker
Apr 12, 2002·The Journal of Biological Chemistry·Bassel Akache, Bernard Turcotte
Mar 8, 2002·The Journal of Biological Chemistry·Karen HellauerBernard Turcotte
Jan 13, 2001·The Journal of Biological Chemistry·X ZhangW S Moye-Rowley
Aug 11, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Anna Kolaczkowska, Andre Goffeau

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