Divergent transcriptional programming of class-specific B cell memory by T-bet and RORα.

Nature Immunology
Nathaniel S WangMichael G McHeyzer-Williams

Abstract

Antibody class defines function in B cell immunity, but how class is propagated into B cell memory remains poorly understood. Here we demonstrate that memory B cell subsets unexpectedly diverged across antibody class through differences in the effects of major transcriptional regulators. Conditional genetic deletion of the gene encoding the transcription factor T-bet selectively blocked the formation and antigen-specific response of memory B cells expressing immunoglobulin G2a (IgG2a) in vivo. Cell-intrinsic expression of T-bet regulated expression of the transcription factor STAT1, steady-state cell survival and transcription of IgG2a-containing B cell antigen receptors (BCRs). In contrast, the transcription factor RORα and not T-bet was expressed in IgA(+) memory B cells, with evidence that knockdown of RORα mRNA expression and chemical inhibition of transcriptional activity also resulted in lower survival and BCR expression of IgA(+) memory B cells. Thus, divergent transcriptional regulators dynamically maintain subset integrity to promote specialized immune function in class-specific memory B cells.

References

May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D A LebmanF D Lee
Apr 5, 2000·The Journal of Experimental Medicine·L J McHeyzer-WilliamsM G McHeyzer-Williams
Jan 29, 2002·Nature Immunology·Stephen W Martin, Christopher C Goodnow
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Stanford L PengLaurie H Glimcher
Aug 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ivan DzhagalovYou-Wen He
May 12, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Karine CrozatBruce Beutler
Dec 23, 2006·Science·Christopher D C AllenJason G Cyster
May 29, 2007·Nature Immunology·Nicolas FazilleauMichael G McHeyzer-Williams
Mar 29, 2008·Annual Review of Immunology·Gislâine Martins, Kathryn Calame
Mar 29, 2008·Annual Review of Immunology·Janet StavnezerCarol E Schrader
Jun 14, 2008·Immunity·Andrea Cerutti, Maria Rescigno
Aug 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Luisa CimminoKathryn L Calame
Mar 3, 2009·Nature Immunology·R Lee ReinhardtRichard M Locksley
Mar 3, 2009·Nature Immunology·Nicolas FazilleauMichael G McHeyzer-Williams
Mar 24, 2009·Immunity·Nicolas FazilleauMichael G McHeyzer-Williams
Apr 4, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zeina JaffarKevan Roberts
Jul 25, 2009·Science·Roza I NurievaChen Dong
Oct 27, 2009·Nature Immunology·Ismail DoganJean-Claude Weill
Mar 3, 2010·Annual Review of Immunology·Sidonia FagarasanKeiichiro Suzuki
Mar 10, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arpita MondalAlexander L Dent
Jun 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yuichi AibaTomohiro Kurosaki
Aug 24, 2010·Immunological Reviews·Tomohiro KurosakiYoshimasa Takahashi
Sep 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Elodie MohrKarine Serre
Nov 3, 2010·Nature Immunology·Nadége PelletierMichael G McHeyzer-Williams
Feb 15, 2011·Annual Review of Immunology·Shane Crotty
Jun 21, 2011·Nature Immunology·Vanja Lazarevic, Laurie H Glimcher
Dec 14, 2011·Nature Reviews. Immunology·Michael McHeyzer-WilliamsLouise McHeyzer-Williams
Dec 17, 2011·Science·Burton E BarnettSteven L Reiner

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Citations

Sep 5, 2012·Immunologic Research·Cristina L SwansonRaul M Torres
Mar 20, 2013·Nature Immunology·Pierre J Milpied, Michael G McHeyzer-Williams
Sep 14, 2013·Science·David Tarlinton, Kim Good-Jacobson
Feb 28, 2013·International Journal of Molecular Sciences·Jun Du, Ren Xu
Jan 28, 2014·International Journal of Medical Microbiology : IJMM·Barbara M BrökerIsabelle Bekeredjian-Ding
Apr 5, 2013·Clinical and Experimental Medicine·Manlio VinciguerraGianluigi Mazzoccoli
Apr 9, 2014·The Journal of Experimental Medicine·Yinan Wang, Deepta Bhattacharya
Feb 28, 2016·Developmental and Comparative Immunology·Irene M Rodríguez-GómezWilhelm Gerner
Apr 14, 2016·The Journal of Experimental Medicine·Shaun W JacksonDavid J Rawlings
Aug 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kira RubtsovaPhilippa Marrack
Feb 14, 2015·Nature Reviews. Immunology·Tomohiro KurosakiWataru Ise
May 27, 2014·Frontiers in Immunology·James J KnoxLaura M McLane
Aug 8, 2013·PloS One·Marc A HogenbirkHeinz Jacobs
Aug 8, 2013·PloS One·Robert D Bremel, E Jane Homan
Apr 1, 2017·Current Opinion in Immunology·Tri Giang Phan, Stuart G Tangye
Mar 11, 2017·Trends in Immunology·Axel Kallies, Kim L Good-Jacobson
Jul 27, 2017·Mucosal Immunology·N Y Lycke, M Bemark
Mar 3, 2018·Immunology and Cell Biology·Beatriz GarcillánFabienne Mackay
Apr 18, 2018·Immunological Reviews·Takeshi InoueTomohiro Kurosaki
Dec 13, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jenna J GuthmillerNoah S Butler
Nov 20, 2013·European Journal of Immunology·Dorottya KövesdiGabriella Sármay
Jul 23, 2014·European Journal of Immunology·Miranda S OakleySanjai Kumar
Aug 10, 2018·The Journal of Clinical Investigation·Alice R BurtonMala K Maini
Oct 26, 2018·European Journal of Immunology·Samuel W DuShaun W Jackson
Nov 30, 2018·Immunology·Rachel Wong, Deepta Bhattacharya
Mar 16, 2019·Immunological Reviews·Scott A JenksIgnacio Sanz
Mar 16, 2019·Immunological Reviews·James J KnoxMichael P Cancro
Sep 26, 2012·Nature Reviews. Immunology·Marc Veldhoen, Verena Brucklacher-Waldert
Oct 17, 2018·The EMBO Journal·Jun Zhi ErJeak Ling Ding
Dec 20, 2015·Immunological Reviews·Martin S NaradikianMichael P Cancro
Aug 5, 2017·Transplant International : Official Journal of the European Society for Organ Transplantation·Sergi LuqueOriol Bestard
Apr 5, 2020·The FEBS Journal·William J OlsonNatascha Hermann-Kleiter
Sep 28, 2017·PLoS Pathogens·Nyamekye Obeng-AdjeiPeter D Crompton

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Methods Mentioned

BETA
transgenic
PCR
nuclear translocation
genotyping

Software Mentioned

Step One
FACSDiva
GraphPad
GraphPad Prism

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