Diverse cytoprotectants prevent cell lysis and promote recovery of respiration and ion transport

Biochemical and Biophysical Research Communications
J H Moran, R G Schnellmann

Abstract

Numerous agents have been reported to prevent cell lysis. However, little information is available concerning the ability of cytoprotectants to promote the return of physiological functions. The goal of this study was to determine whether a diverse group of cytoprotectants prevent cell lysis and promote the recovery of respiration and ion transport following anoxia (60 min)/reoxygenation (60 min) in rabbit renal proximal tubule (RPT) suspensions. Cell lysis (LDH release) was determined immediately following the anoxic and reoxygenation periods. Mitochondrial function (basal respiration) and active Na+ transport (ouabain-sensitive respiration) was determined after the reoxygenation period. LDH release increased to 75 +/- 11% after the anoxic period and did not increase further during the reoxygenation period. LDH release in controls was 6 +/- 1% and did not vary over time. Glycine (2 mM), strychnine (1 mM), nifedipine (100 microM) and niflumic acid (100 microM) added immediately prior to the anoxic period completely blocked LDH release. All cytoprotectants increased basal respiration from 39 +/- 7% of controls in the anoxic samples to 65-77% of controls. Glycine, strychnine and nifedipine increased ouabain-sensitive respiration ...Continue Reading

References

Sep 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D P RodeheaverR G Schnellmann
Feb 1, 1990·The Journal of Clinical Investigation·L J MandelW R Jacobs
Jun 1, 1990·The American Journal of Physiology·J M WeinbergR Kunkel
Jan 1, 1994·In Vitro Cellular & Developmental Biology. Animal·R D Griner, R G Schnellmann
Sep 1, 1996·Journal of Pharmacological and Toxicological Methods·J H Moran, R G Schnellmann

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Citations

Jan 28, 2004·Annual Review of Pharmacology and Toxicology·Xiuli LiuRick G Schnellmann
Aug 12, 2014·BioMed Research International·Guang ZhangYitao Ding
Nov 21, 1997·Biochemical and Biophysical Research Communications·S L WatersR G Schnellmann
Feb 22, 2001·Toxicology and Applied Pharmacology·J ChenR G Schnellmann
Mar 21, 1998·Toxicologic Pathology·S L Waters, R G Schnellmann
Jan 25, 2008·American Journal of Physiology. Renal Physiology·Lawrence H Lash
Mar 18, 2010·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Tamas BenkoUrsula Rauen
Jun 18, 2002·The Journal of Pharmacology and Experimental Therapeutics·Xiuli LiuRick G Schnellmann
Sep 13, 2001·American Journal of Physiology. Renal Physiology·X LiuR G Schnellmann
Apr 14, 2016·Cellular and Molecular Life Sciences : CMLS·Joel M WeinbergM A Venkatachalam

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