Diverse Genetic Array of blaCTXM-15 in Escherichia coli: A Single-Center Study from India

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Anand Prakash MauryaAmitabha Bhattacharjee

Abstract

CTX-M-15 is a chief contributor for expanded-spectrum cephalosporin and monobactam resistance in India, complicating treatment options. In this study, we have investigated genetic context of CTX-M-15 in Escherichia coli and their transmission dynamics in a tertiary referral hospital of India. A total of 198 isolates were collected, of which 66 were harboring blaCTXM-15. Among them, 14 isolates were carrying a single CTX-M-15 gene and 52 were harboring multiple extended-spectrum β-lactamase genes along with blaCTX-M-15. The resistance gene was flanked by tnpA, ISEcp1, IS26, and ORF477 in 10 different arrangements. The resistance determinant was horizontally transferable through F, W, I1, and P Inc types of plasmids. Restriction mapping of plasmids showed a variable band pattern even within the same Inc types. Minimum inhibitory concentration was found above the breakpoint level against expanded-spectrum cephalosporins and monobactam while susceptible against carbapenems. blaCTX-M-15 was highly stable and sustained in the cell after 115 serial passages. In pulse-field gel electrophoresis, eight pulsotypes of E. coli were found to be responsible for the spread of blaCTX-M-15 in the tertiary referral center. We conclude that the pr...Continue Reading

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Citations

Apr 15, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Varsha Rani GajamerHare Krishna Tiwari

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Methods Mentioned

BETA
electrophoresis
PCR

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