Diverse Hormone Response Networks in 41 Independent Drosophila Cell Lines

G3 : Genes - Genomes - Genetics
Marcus StoiberP Cherbas

Abstract

Steroid hormones induce cascades of gene activation and repression with transformative effects on cell fate . Steroid transduction plays a major role in the development and physiology of nearly all metazoan species, and in the progression of the most common forms of cancer. Despite the paramount importance of steroids in developmental and translational biology, a complete map of transcriptional response has not been developed for any hormone . In the case of 20-hydroxyecdysone (ecdysone) in Drosophila melanogaster, these trajectories range from apoptosis to immortalization. We mapped the ecdysone transduction network in a cohort of 41 cell lines, the largest such atlas yet assembled. We found that the early transcriptional response mirrors the distinctiveness of physiological origins: genes respond in restricted patterns, conditional on the expression levels of dozens of transcription factors. Only a small cohort of genes is constitutively modulated independent of initial cell state. Ecdysone-responsive genes tend to organize into directional same-stranded units, with consecutive genes induced from the same strand. Here, we identify half of the ecdysone receptor heterodimer as the primary rate-limiting step in the response, and...Continue Reading

References

Oct 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K UiT Miyake
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P CherbasK Nakanishi
Apr 1, 1994·In Vitro Cellular & Developmental Biology. Animal·K UiT Miyake
Aug 30, 1995·Molecular and Cellular Endocrinology·P P D'AvinoM Furia
Jul 11, 2003·Developmental Cell·Tong-Ruei Li, Kevin P White
Dec 17, 2005·Genome Biology·Robert B BecksteadCarl S Thummel
Mar 7, 2006·Cell Motility and the Cytoskeleton·Nina KarpovaAlain Debec
Aug 31, 2006·Methods in Enzymology·Brian EadsJusten Andrews
Sep 23, 2006·The Journal of Biological Chemistry·Claudia B ZralyAndrew K Dingwall
Oct 4, 2006·Molecular and Cellular Biology·Jinsong ZhuAlexander S Raikhel
Oct 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yuzo NikiAnthony P Mahowald
Feb 28, 2007·Genome Biology·Shobhit GuptaWilliam Stafford Noble
Feb 25, 2009·Genome Research·Zareen GauharKevin P White
May 12, 2009·Molecular and Cellular Endocrinology·Anca Azoitei, Margarethe Spindler-Barth
Sep 15, 2009·Archives of Insect Biochemistry and Physiology·Simone BraunMargarethe Spindler-Barth
Oct 3, 2009·The FEBS Journal·Yoshiaki Nakagawa, Vincent C Henrich
Dec 31, 2009·Genes & Development·Shun SawatsubashiShigeaki Kato
Mar 23, 2010·Journal of Insect Physiology·Ann M Fallon, Anna Gerenday
Jul 30, 2010·Neuroscience Letters·Makoto TominagaHiroyuki Nakagawa
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
Dec 24, 2010·Genome Research·Lucy CherbasPeter Cherbas
Apr 18, 2012·Genome Research·Wendy A KellnerVictor G Corces
Jun 23, 2012·Genome Research·Simon AndersWolfgang Huber

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Citations

May 26, 2017·Genes & Development·Christopher M UyeharaDaniel J McKay
Nov 2, 2016·Biology Open·Yongfeng GuoLaura A Buttitta
Dec 19, 2018·Wiley Interdisciplinary Reviews. Developmental Biology·Arthur LuhurAndrew C Zelhof
Mar 8, 2020·Genome Research·Logan J EverettTrudy F C Mackay
May 2, 2017·Current Stem Cell Reports·Elizabeth T Ables, Daniela Drummond-Barbosa
May 27, 2016·G3 : Genes - Genomes - Genetics·Elizabeth T AblesDaniela Drummond-Barbosa
Apr 26, 2019·Proceedings of the National Academy of Sciences of the United States of America·Christopher M Uyehara, Daniel J McKay
Mar 12, 2021·G3 : Genes - Genomes - Genetics·Allison N BeachumElizabeth T Ables
Jun 4, 2021·Developmental Cell·Torsten U BanischRuth Lehmann

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Datasets Mentioned

BETA
PRJNA306537
GSE11167

Methods Mentioned

BETA
acetylation
RNA-seq
Hi-seq

Software Mentioned

R package
LOESS
homer2
DESeq
HTSeq
DESeq R
R
DEXSeq R package
DESeq R package

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