Diverse Mechanisms and Circuitry for Global Regulation by the RNA-Binding Protein CsrA.

Frontiers in Microbiology
Christine PourciauTony Romeo

Abstract

The carbon storage regulator (Csr) or repressor of stationary phase metabolites (Rsm) system of Gammaproteobacteria is among the most complex and best-studied posttranscriptional regulatory systems. Based on a small RNA-binding protein, CsrA and homologs, it controls metabolism, physiology, and bacterial lifestyle decisions by regulating gene expression on a vast scale. Binding of CsrA to sequences containing conserved GGA motifs in mRNAs can regulate translation, RNA stability, riboswitch function, and transcript elongation. CsrA governs the expression of dozens of transcription factors and other regulators, further expanding its influence on cellular physiology, and these factors can participate in feedback to the Csr system. Expression of csrA itself is subject to autoregulation via translational inhibition and indirect transcriptional activation. CsrA activity is controlled by small noncoding RNAs (sRNAs), CsrB and CsrC in Escherichia coli, which contain multiple high affinity CsrA binding sites that compete with those of mRNA targets. Transcription of CsrB/C is induced by certain nutrient limitations, cellular stresses, and metabolites, while these RNAs are targeted for degradation by the presence of a preferred carbon sou...Continue Reading

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Citations

Jul 11, 2021·Molecular Microbiology·Thomas Søndergaard Stenum, Erik Holmqvist
Jun 10, 2021·Molecular Microbiology·Ying-Jung LaiTony Romeo
Aug 28, 2021·Frontiers in Microbiology·Brice Felden, Yoann Augagneur

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Methods Mentioned

BETA
exponential enrichment
SELEX
nuclear magnetic resonance
NMR
electron paramagnetic resonance
immunoprecipitation
CLIP-seq
RNA-seq

Software Mentioned

PyMol

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