Diversifying Selection Analysis Predicts Antigenic Evolution of 2009 Pandemic H1N1 Influenza A Virus in Humans

Journal of Virology
Alexandra J LeeRichard H Scheuermann

Abstract

Although a large number of immune epitopes have been identified in the influenza A virus (IAV) hemagglutinin (HA) protein using various experimental systems, it is unclear which are involved in protective immunity to natural infection in humans. We developed a data mining approach analyzing natural H1N1 human isolates to identify HA protein regions that may be targeted by the human immune system and can predict the evolution of IAV. We identified 16 amino acid sites experiencing diversifying selection during the evolution of prepandemic seasonal H1N1 strains and found that 11 sites were located in experimentally determined B-cell/antibody (Ab) epitopes, including three distinct neutralizing Caton epitopes: Sa, Sb, and Ca2 [A. J. Caton, G. G. Brownlee, J. W. Yewdell, and W. Gerhard, Cell 31:417-427, 1982, http://dx.doi.org/10.1016/0092-8674(82)90135-0]. We predicted that these diversified epitope regions would be the targets of mutation as the 2009 H1N1 pandemic (pH1N1) lineage evolves in response to the development of population-level protective immunity in humans. Using a chi-squared goodness-of-fit test, we identified 10 amino acid sites that significantly differed between the pH1N1 isolates and isolates from the recent 2012-...Continue Reading

References

Mar 1, 1992·Microbiological Reviews·R G WebsterY Kawaoka
Jan 1, 1984·Journal of Molecular Evolution·C LanaveG Serio
Feb 14, 1997·The Journal of Biological Chemistry·S Y Mir-ShekariI T Schulze
Oct 29, 2004·Bioinformatics·Sergei L Kosakovsky PondSpencer V Muse
Nov 11, 2009·Science·Scott E HensleyJonathan W Yewdell
Nov 13, 2009·Nucleic Acids Research·Randi VitaBjoern Peters
Jun 5, 2010·Avian Diseases·Ron A M Fouchier, Derek J Smith
Mar 23, 2011·Journal of Virological Methods·E M AbdelwhabHafez M Hafez
Sep 3, 2011·PloS One·Monica GalianoMaria Zambon
Jan 21, 2012·Influenza and Other Respiratory Viruses·R Burke SquiresRichard H Scheuermann
Apr 25, 2012·Methods in Molecular Biology·Bin Zhou, David E Wentworth
Feb 20, 2013·Molecular Biology and Evolution·Ben MurrellKonrad Scheffler
May 17, 2013·Science Translational Medicine·Philip R DormitzerJ Craig Venter
Nov 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nicholas S HeatonPeter Palese

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Citations

Jun 5, 2016·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Moataz Abd El GhanyGrant A Hill-Cawthorne
Feb 13, 2018·BMC Bioinformatics·Christopher S AndersonDavid J Topham
Jan 31, 2019·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Danuta M SkowronskiGaston De Serres
Jul 12, 2018·Scientific Reports·Ericka KirkpatrickFlorian Krammer
Mar 5, 2019·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Jeeyong KimChae Seung Lim

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