Abstract
Although a large number of immune epitopes have been identified in the influenza A virus (IAV) hemagglutinin (HA) protein using various experimental systems, it is unclear which are involved in protective immunity to natural infection in humans. We developed a data mining approach analyzing natural H1N1 human isolates to identify HA protein regions that may be targeted by the human immune system and can predict the evolution of IAV. We identified 16 amino acid sites experiencing diversifying selection during the evolution of prepandemic seasonal H1N1 strains and found that 11 sites were located in experimentally determined B-cell/antibody (Ab) epitopes, including three distinct neutralizing Caton epitopes: Sa, Sb, and Ca2 [A. J. Caton, G. G. Brownlee, J. W. Yewdell, and W. Gerhard, Cell 31:417-427, 1982, http://dx.doi.org/10.1016/0092-8674(82)90135-0]. We predicted that these diversified epitope regions would be the targets of mutation as the 2009 H1N1 pandemic (pH1N1) lineage evolves in response to the development of population-level protective immunity in humans. Using a chi-squared goodness-of-fit test, we identified 10 amino acid sites that significantly differed between the pH1N1 isolates and isolates from the recent 2012-...Continue Reading
References
Mar 1, 1992·Microbiological Reviews·R G WebsterY Kawaoka
Dec 1, 1982·Cell·A J CatonW Gerhard
Jan 1, 1984·Journal of Molecular Evolution·C LanaveG Serio
Feb 14, 1997·The Journal of Biological Chemistry·S Y Mir-ShekariI T Schulze
Oct 29, 2004·Bioinformatics·Sergei L Kosakovsky PondSpencer V Muse
Dec 21, 2004·Bioinformatics·A StamatakisH Meier
Jul 17, 2009·Journal of Virology·Bin ZhouDavid E Wentworth
Nov 11, 2009·Science·Scott E HensleyJonathan W Yewdell
Nov 13, 2009·Nucleic Acids Research·Randi VitaBjoern Peters
Nov 20, 2009·PloS One·Jun ShenQinghua Wang
Jun 5, 2010·Avian Diseases·Ron A M Fouchier, Derek J Smith
Jul 6, 2010·Virology·Yuki FuruseHitoshi Oshitani
Nov 12, 2010·Journal of Virology·Rebecca M DuBoisCharles J Russell
Mar 23, 2011·Journal of Virological Methods·E M AbdelwhabHafez M Hafez
Apr 22, 2011·Virology Journal·Wenfu LiChaodong Zhu
Sep 3, 2011·PloS One·Monica GalianoMaria Zambon
Jan 21, 2012·Influenza and Other Respiratory Viruses·R Burke SquiresRichard H Scheuermann
Mar 2, 2012·Journal of Virology·Nitin VermaSurender Khurana
Mar 9, 2012·Journal of Virology·Jyothi M NoronhaRichard H Scheuermann
Apr 25, 2012·Methods in Molecular Biology·Bin Zhou, David E Wentworth
Aug 14, 2012·Vaccine·Jhang-Wei HuangJinn-Moon Yang
Nov 14, 2012·The Journal of Infectious Diseases·Jennifer L NayakAndrea J Sant
Nov 16, 2012·PloS One·Gianguglielmo ZehenderAlessandro Remo Zanetti
Dec 20, 2012·PloS One·Jarika MakkochYong Poovorawan
Feb 20, 2013·Molecular Biology and Evolution·Ben MurrellKonrad Scheffler
Mar 19, 2013·Cell Host & Microbe·Suman R DasJonathan W Yewdell
May 17, 2013·Science Translational Medicine·Philip R DormitzerJ Craig Venter
Aug 1, 2013·Immunogenetics·Edita KarosieneMorten Nielsen
Nov 12, 2013·Virology·B E PickettR H Scheuermann
Nov 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nicholas S HeatonPeter Palese
Citations
Nov 29, 2015·Genome Biology and Evolution·Sergei S BelanovDenis E Kainov
Jun 5, 2016·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Moataz Abd El GhanyGrant A Hill-Cawthorne
Nov 18, 2017·PloS One·Amelia M ClarkDavid J Topham
Feb 13, 2018·BMC Bioinformatics·Christopher S AndersonDavid J Topham
Aug 8, 2015·Nature Communications·Yvonne C F SuGavin J D Smith
Dec 9, 2017·PloS One·Jose Reyes Canche-PechGuadalupe Ayora-Talavera
Mar 29, 2018·Viruses·Nastasja C HauckI-Na Lu
Jan 31, 2019·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Danuta M SkowronskiGaston De Serres
Jul 12, 2018·Scientific Reports·Ericka KirkpatrickFlorian Krammer
Jul 7, 2021·Heliyon·Ron S Hirschprung, Chen Hajaj
Aug 28, 2021·Human Gene Therapy·Qiang WangJames M Wilson
Mar 5, 2019·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Jeeyong KimChae Seung Lim