Diversity among Field Populations of Bradyrhizobium japonicum in Poland.

Applied and Environmental Microbiology
C J MadrzakMichael J Sadowsky

Abstract

Genetic structure in field populations of Bradyrhizobium japonicum isolated in Poland was determined by using several complementary techniques. Of the 10 field sites examined, only 4 contained populations of indigenous B. japonicum strains. The Polish bradyrhizobia were divided into at least two major groups on the basis of protein profiles on polyacrylamide gels, serological reaction with polyclonal antisera, repetitive extragenic palindromic PCR fingerprints of genomic DNA, and Southern hybridization analyses with nif and nod gene probes. Serological analyses indicated that 87.5% of the Polish B. japonicum isolates tested were in serogroups 123 and 129, while seven (12.5%) of the isolates tested belonged to their own unique serogroup. These seven strains also could be grouped together on the basis of repetitive extragenic palindromic PCR fingerprints, protein profiles, and Southern hybridization analyses. Cluster analyses indicated that the seven serologically undefined isolates were genetically dissimilar from the majority of the Polish B. japonicum strains. Moreover, immuno-cross-adsorption studies indicated that although the Polish B. japonicum strains reacted with polyclonal antisera prepared against strain USDA123, the m...Continue Reading

References

Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M J SadowskyG Stacey
Jul 1, 1985·Journal of Bacteriology·J StanleyD P Verma
Jun 1, 1968·Journal of Bacteriology·E L SchmidtB B Bohlool
Nov 1, 1969·Archives of Biochemistry and Biophysics·M Steinbuch, R Audran
Jun 1, 1971·Applied Microbiology·W F Dudman
Feb 1, 1965·Canadian Journal of Microbiology·R A DATE, A M DECKER
Nov 1, 1980·Applied and Environmental Microbiology·K D Noel, W J Brill
Feb 1, 1983·Applied and Environmental Microbiology·F M Robert, E L Schmidt
May 1, 1986·Applied and Environmental Microbiology·P W Singleton, J W Tavares
Jun 1, 1986·Applied and Environmental Microbiology·E L SchmidtH M Abebe
Aug 1, 1987·Applied and Environmental Microbiology·M J SadowskyH H Keyser
Sep 1, 1993·Applied and Environmental Microbiology·P van BerkumJ S Angle

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Citations

Jan 15, 2013·International Journal of Systematic and Evolutionary Microbiology·Shen ZhouXiaoping Zhang
Dec 17, 2009·International Journal of Systematic and Evolutionary Microbiology·Ping Fa ZhouGe Hong Wei
Jun 7, 2015·Applied and Environmental Microbiology·Dorota NarożnaMichael J Sadowsky
Dec 14, 2005·Applied and Environmental Microbiology·M SantamaríaJ Corzo
Jun 22, 2021·Frontiers in Plant Science·Mosab HalwaniRalf Bloch

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