Diversity and Complexity of the Large Surface Protein Family in the Compacted Genomes of Multiple Pneumocystis Species.

MBio
Liang MaJoseph A Kovacs

Abstract

Pneumocystis, a major opportunistic pathogen in patients with a broad range of immunodeficiencies, contains abundant surface proteins encoded by a multicopy gene family, termed the major surface glycoprotein (Msg) gene superfamily. This superfamily has been identified in all Pneumocystis species characterized to date, highlighting its important role in Pneumocystis biology. In this report, through a comprehensive and in-depth characterization of 459 msg genes from 7 Pneumocystis species, we demonstrate, for the first time, the phylogeny and evolution of conserved domains in Msg proteins and provide a detailed description of the classification, unique characteristics, and phylogenetic relatedness of five Msg families. We further describe, for the first time, the relative expression levels of individual msg families in two rodent Pneumocystis species, the substantial variability of the msg repertoires in P. carinii from laboratory and wild rats, and the distinct features of the expression site for the classic msg genes in Pneumocystis from 8 mammalian host species. Our analysis suggests multiple functions for this superfamily rather than just conferring antigenic variation to allow immune evasion as previously believed. This stud...Continue Reading

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Citations

Jun 21, 2020·The Journal of Infectious Diseases·Emanuel Schmid-SiegertPhilippe M Hauser
Mar 10, 2021·Communications Biology·Ousmane H CisséJoseph A Kovacs
Jun 17, 2021·Microbiology and Molecular Biology Reviews : MMBR·Philippe M Hauser

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Datasets Mentioned

BETA
PRJNA223519
JX499145

Methods Mentioned

BETA
reverse transcription-PCR
PCR
RNA-Seq
Illumina sequencing

Software Mentioned

bowtie
MUSCLE
RSEM
RAxML

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