Diversity and distribution of Maize-associated totivirus strains from Tanzania

Virus Genes
David Alan ReadErnest R Mbega

Abstract

Typically associated with fungal species, members of the viral family Totiviridae have recently been shown to be associated with plants, including important crop species, such as Carica papaya (papaya) and Zea mays (maize). Maize-associated totivirus (MATV) was first described in China and more recently in Ecuador, where it has been found to co-occur with other viruses known to elicit maize lethal necrosis disease (MLND). In a survey for maize-associated viruses, 35 samples were selected for Illumina HiSeq sequencing, from the Tanzanian maize producing regions of Mara, Arusha, Manyara, Kilimanjaro, Morogoro and Pwani. Libraries were prepared using an RNA-tag-seq methodology. Taxonomic classification of the resulting datasets showed that 6 of the 35 samples from the regions of Arusha, Kilimanjaro, Morogoro and Mara, contained reads that were assigned to MATV reference sequences. This was confirmed with PCR and Sanger sequencing. Read assembly of the six MATV-associated datasets yielded partial MATV genomes, two of which were selected for further characterization, using RACE. This yielded two full-length MATV genomes, one of which is divergent from other available MATV genomes.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Mar 11, 2006·The Journal of General Virology·Bonnie T PoulosDonald V Lightner
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Mar 3, 2015·Nature Methods·Alexander A ShishkinJonathan Livny
Mar 17, 2015·Virology·Said A GhabrialNobuhiro Suzuki
Nov 13, 2015·Archives of Virology·Sha ChenXueping Zhou
Apr 14, 2016·Nature Communications·Peter MenzelAnders Krogh
May 12, 2016·PloS One·Tathiana Ferreira Sá AntunesPatricia Machado Bueno Fernandes
Nov 24, 2016·Acta Tropica·Amaranta Gómez-ArreazaLuisana Avilán
Dec 3, 2016·Archives of Virology·Robert A Alvarez-QuintoDiego F Quito-Avila
Nov 9, 2017·PloS One·Andressa Zelenski de Lara PintoRenata Dezengrini Slhessarenko
Jul 31, 2018·Annual Review of Virology·Margaret G Redinbaugh, Lucy R Stewart
Aug 8, 2018·The New Phytologist·Marilyn J Roossinck

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Datasets Mentioned

BETA
MK066242
MK066243

Methods Mentioned

BETA
RNA-tag-seq

Software Mentioned

MEGA
BioEdit
BLASTx
Kaiju
CLC Genomics Workbench
Contig Assembly Program ( CAP )

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