Diversity and evolution of cytochrome P450s of Jacobaea vulgaris and Jacobaea aquatica.

BMC Plant Biology
Yangan ChenKlaas Vrieling

Abstract

Collectively, plants produce a huge variety of secondary metabolites (SMs) which are involved in the adaptation of plants to biotic and abiotic stresses. The most characteristic feature of SMs is their striking inter- and intraspecific chemical diversity. Cytochrome P450 monooxygenases (CYPs) often play an important role in the biosynthesis of SMs and thus in the evolution of chemical diversity. Here we studied the diversity and evolution of CYPs of two Jacobaea species which contain a characteristic group of SMs namely the pyrrolizidine alkaloids (PAs). We retrieved CYPs from RNA-seq data of J. vulgaris and J. aquatica, resulting in 221 and 157 full-length CYP genes, respectively. The analyses of conserved motifs confirmed that Jacobaea CYP proteins share conserved motifs including the heme-binding signature, the PERF motif, the K-helix and the I-helix. KEGG annotation revealed that the CYPs assigned as being SM metabolic pathway genes were all from the CYP71 clan but no CYPs were assigned as being involved in alkaloid pathways. Phylogenetic analyses of full-length CYPs were conducted for the six largest CYP families of Jacobaea (CYP71, CYP76, CYP706, CYP82, CYP93 and CYP72) and were compared with CYPs of two other members of ...Continue Reading

References

Oct 25, 1990·Nucleic Acids Research·T D Schneider, R M Stephens
Jul 7, 1995·Journal of Theoretical Biology·G J Graham
Jul 13, 2000·Journal of Molecular Biology·O EmanuelssonG von Heijne
Jun 13, 2001·Plant Physiology·D J KliebensteinT Mitchell-Olds
Aug 9, 2002·Theoretical Population Biology·Jonathan A Eisen, Martin Wu
Jun 26, 2003·Nucleic Acids Research·Elisabeth GasteigerAmos Bairoch
Apr 15, 2004·Phytochemistry·Mirka MacelPeter G L Klinkhamer
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
May 29, 2007·Nucleic Acids Research·Yuki MoriyaMinoru Kanehisa
Feb 7, 2008·Annual Review of Plant Biology·Jörg Ziegler, Peter J Facchini
Nov 19, 2008·Nature Reviews. Genetics·Gavin C Conant, Kenneth H Wolfe
May 22, 2009·Nucleic Acids Research·Timothy L BaileyWilliam S Noble
Jul 22, 2009·Nucleic Acids Research·Dmitri ParkhomchukAlexey Soldatov
Dec 3, 2009·Human Genomics·David R Nelson
Mar 3, 2010·Annual Review of Plant Biology·Masaharu Mizutani, Daisaku Ohta
Mar 30, 2011·The Plant Journal : for Cell and Molecular Biology·David Nelson, Danièle Werck-Reichhart
Apr 2, 2011·The Journal of Biological Chemistry·Lesley-Ann GiddingsSarah E O'Connor
Jan 1, 2002·The Arabidopsis Book·Danièle Werck-ReichhartSuzanne Paquette
Feb 4, 2012·The Arabidopsis Book·Søren BakDanièle Werck-Reichhart
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Jun 13, 2012·Biological & Pharmaceutical Bulletin·Masaharu Mizutani
Oct 13, 2012·Bioinformatics·Limin FuWeizhong Li
Jan 9, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Björn Hamberger, Søren Bak
Oct 15, 2013·The New Phytologist·Ben D MooreWilliam J Foley
Nov 30, 2013·Nucleic Acids Research·Robert D FinnMarco Punta
Nov 6, 2014·Molecular Biology and Evolution·Lam-Tung NguyenBui Quang Minh
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Apr 12, 2017·Bioinformatics·Sebastian BeierMartin Mascher
Apr 13, 2017·Nature Communications·Sebastian Reyes-Chin-WoRichard W Michelmore
Apr 30, 2017·Methods in Molecular Biology·Henrik Nielsen
May 10, 2017·Nature Methods·Subha KalyaanamoorthyLars S Jermiin
Sep 8, 2017·Frontiers in Plant Science·Weifang LiaoLongjiang Yu

❮ Previous
Next ❯

Citations

Sep 9, 2021·Journal of Agricultural and Food Chemistry·Yuanpeng FangXin Xie

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA561604

Methods Mentioned

BETA
Illumina sequencing
RNAseq
chip
PCR

Software Mentioned

KEGG Automatic Annotation Server ( KAAS )
FastQC
Multiple Expectation Maximization for Motif Elicitation ( MEME )
IQ
TMHMM
Trimmomatic
BLASTp
HIT
Trinotate
Bowtie2

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Antioxidants
Balaji Aravindhan PandianMithila Jugulam
International Journal of Biological Macromolecules
Archana SinghIndrakant Kumar Singh
Frontiers in Bioscience : a Journal and Virtual Library
Tsunehiro OyamaToshihiro Kawamoto
© 2021 Meta ULC. All rights reserved